Dopamine release induced by electrical field stimulation of rat hypothalamus invitro: Inhibition by melatonin

Nava Zisapel*, Moshe Laudon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Microdissected slices of rat hypothalamus were incubated with 3H -dopamine and then subjected to two successive sets of electrical field stimulation in a superfusion chamber. Neurotransmitter release was found to be calcium dependent and the amount of release was determined by scintillation counting of the effluent buffer. The release obtained following the first train of stimuli served as an internal reference. The samples were exposed to drugs during the interval between the two sets of stimuli. Using this technique, as well as K+-evoked depolarization, we were able to show that subnanomolar concentrations of melatonin, the hormone secreted from the pineal gland, inhibits dopamine release from hypothalamic slices. The possibility that melatonin modulates neurotransmission in the brain is therefore indicated.

Original languageEnglish
Pages (from-to)1610-1616
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume104
Issue number4
DOIs
StatePublished - 26 Feb 1982

Fingerprint

Dive into the research topics of 'Dopamine release induced by electrical field stimulation of rat hypothalamus invitro: Inhibition by melatonin'. Together they form a unique fingerprint.

Cite this