@article{0acb948fbbaf4e7c97aaf524f19f5abd,
title = "Dopamine induces apoptosis-like cell death in cultured chick sympathetic neurons - A possible novel pathogenetic mechanism in Parkinson's disease",
abstract = "We report that exposure of cultured, postmitotic chick-embryo sympathetic neurons, to physiological concentrations of dopamine (0.1-1 mM) for 24 h initiates a cellular death process characteristic of apoptosis (= programmed-cell-death, PCD). Dopamine caused marked morphological alterations, mainly axonal disintegration and severe shrinkage and condensation of cell bodies. Flow-cytometric analysis of propidium-iodide-stained cell nuclei revealed the characteristic apoptotic nuclear fragmentation: increase in nuclear granularity and emergence of a large, distinct population of nuclei with reduced DNA content (subdiploid, apoptotic peak). These alterations were similar to changes induced by nerve growth factor (NGF) deprivation, a model of sympathetic neuronal PCD. Alterations were inhibited by the anti-oxidative agent DTT. Inappropriate, dopamine-induced activation of PCD might have a role in nigral neuronal degeneration in Parkinson's disease.",
keywords = "Apoptosis, DNA damage, Dopamine, Flow-cytometry, Free radical, Parkinson's disease",
author = "Ilan Ziv and Eldad Melamed and Nurit Nardi and Drorit Luria and Anat Achiron and Daniel Offen and Ari Barzilai",
note = "Funding Information: Supported, in part, by Teva Pharmaceutical Industries, Ltd, the National Parkinson Foundation, Miami, Florida, USA and the National Institute of Psychobiology, Israel. We also acknowledge the great assistance of Ruth Oren, from the Hebrew University, Jerusalem, in the morphological studies.",
year = "1994",
month = mar,
day = "28",
doi = "10.1016/0304-3940(94)90258-5",
language = "אנגלית",
volume = "170",
pages = "136--140",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",
}