Donation of information to the unbroken chromosome in double-strand break repair

Chaim Roigrund, Rivka Steinlauf, Martin Kupiec

Research output: Contribution to journalArticlepeer-review

Abstract

We have used transformation of yeast with lincarized plasmids to study the transfer of information to the unbroken chromosome during double-strand break repair. Using a strain which carried the wild-type HIS3 allele, and a linearized plasmid which carried a mutant his3 allele, we have obtained His- transformants. In these, double-strand break repair has resulted in precise transfer of genetic information from the plasmid to the chromosome. Such repair events, we suggest, are gene conversions which entail the formation of heteroduplex DNA on the (unbroken) chromosome. If this suggestion is correct, our results reflect the spatial distribution of such heteroduplex DNA. Transfer of information from the plasmid to the chromosome was obtained at a maximal frequency of 1.5% of the repair events, and showed a dependence with distance. Transformation to His- was also obtained with a 2-kbp insertion and with a deletion of 200 bp. The latter results suggest that gene conversion of large heterologies can occur via repair of a heteroduplex DNA intermediate.

Original languageEnglish
Pages (from-to)414-422
Number of pages9
JournalCurrent Genetics
Volume23
Issue number5-6
DOIs
StatePublished - Sep 1993

Keywords

  • Donation
  • Double-strand break repair
  • Gene conversion
  • Heteroduplex DNA
  • Saccharomyces cerevisiae

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