@article{acba7b7966e946d3bb236c7b7374fc5c,
title = "DNA sequences amplified in cancer cells: an interface between tumor biology and human genome analysis",
abstract = "There is growing evidence that amplification of specific genes is associated with tumor progression. While several proto-oncogenes are known to be activated by amplification, it is clear that not all the genes involved in DNA amplification in human tumors have been discovered. Our approach to the identification of such genes is based on the 'reverse genetics' methodology. Anonymous amplified DNA fragments are cloned by virtue of their amplification in a given tumor. These sequences are mapped in the normal genome and hence define a new genetic locus. The amplified domain is isolated by long-range cloning and analyzed along three lines of investigation: new genes are sought that can explain the biological significance of the amplification; the structure of the domain is studied in normal cells and in the amplification unit in the cancer cell; attempts are made to identify molecular probes of diagnostic value within the amplified domain. This application of genome technology to cancer biology is demon-strated in our study of a new genomic domain at chromosome 10q26 which is amplified specifically in human gastric carcinomas.",
keywords = "Cancer, DNA amplification, Human genome, Molecular oncology, Oncogenes",
author = "Yosef Shiloh and Orna Mor and Ari Manor and Irit Bar-Am and Galit Rotman and James Eubanks and Mordechai Gutman and Ranzani, {Guglielmina N.} and Jane Houldsworth and Glen Evans and Lydia Avivi",
note = "Funding Information: We wish to thank Dr. Adi F. Gazdar of the National Cancer Institute (U.S.A.) and Dr. J.-G. Park of Seoul National University (Korea) for the cell line SNU16, Dr. Rima Rozen of McGill University (Canada) for the ornithine amino- transferase probe and Adam Sartiel for art work. This study was supported by a joint research grant from the Deutsches Krebsforschungszen-trum (DKFZ) and the Israel National Council for Research and Development.",
year = "1992",
month = may,
doi = "10.1016/0165-1110(92)90019-6",
language = "אנגלית",
volume = "276",
pages = "329--337",
journal = "Mutation Research - Reviews in Genetic Toxicology",
issn = "0165-1110",
publisher = "Elsevier BV",
number = "3",
}
