DNA polymerase epsilon may be dispensable for SV40- but not cellular-DNA replication

Tamar Zlotkin, Gabriel Kaufmann, Yunquan Jiang, Marietta Y.W.T. Lee, Lahja Uitto, Juhani Syväoja, Irena Dornreiter, Ellen Fanning, Tamar Nethanel

Research output: Contribution to journalArticlepeer-review

Abstract

The contributions of DNA polymerases α, δ and ε to SV40 and nuclear DNA syntheses were evaluated. Proteins were UV-crosslinked to nascent DNA within replicating chromosomes and the photolabelled polymerases were immunopurified. Only DNA polymerases α and δ were detectably photolabelled by nascent SV40 DNA, whether synthesized in soluble viral chromatin or within nuclei isolated from SV40-infected cells. In contrast, all three enzymes were photolabelled by the nascent cellular DNA. Mitogenic stimulation enhanced the photolabelling of the polymerases in the α>δ>ε order of preference. The data agree with the notion that DNA polymerases α and δ catalyse the principal DNA polymerisation reactions at the replication fork of SV40 and, perhaps, also of nuclear chromosomes. DNA polymerase ε, implicated by others as a cell-cycle checkpoint regulator sensing DNA replication lesions, may be dispensable for replication of the small, fast propagating virus that subverts cell cycle controls.

Original languageEnglish
Pages (from-to)2298-2305
Number of pages8
JournalEMBO Journal
Volume15
Issue number9
DOIs
StatePublished - 1 May 1996

Keywords

  • Aphidicolin
  • Butylphenyl-dGTP
  • Cell cycle checkpoint
  • DNA polymerases α and δ
  • Protein-DNA UV-crosslinking

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