TY - JOUR
T1 - DNA polymerase epsilon may be dispensable for SV40- but not cellular-DNA replication
AU - Zlotkin, Tamar
AU - Kaufmann, Gabriel
AU - Jiang, Yunquan
AU - Lee, Marietta Y.W.T.
AU - Uitto, Lahja
AU - Syväoja, Juhani
AU - Dornreiter, Irena
AU - Fanning, Ellen
AU - Nethanel, Tamar
PY - 1996/5/1
Y1 - 1996/5/1
N2 - The contributions of DNA polymerases α, δ and ε to SV40 and nuclear DNA syntheses were evaluated. Proteins were UV-crosslinked to nascent DNA within replicating chromosomes and the photolabelled polymerases were immunopurified. Only DNA polymerases α and δ were detectably photolabelled by nascent SV40 DNA, whether synthesized in soluble viral chromatin or within nuclei isolated from SV40-infected cells. In contrast, all three enzymes were photolabelled by the nascent cellular DNA. Mitogenic stimulation enhanced the photolabelling of the polymerases in the α>δ>ε order of preference. The data agree with the notion that DNA polymerases α and δ catalyse the principal DNA polymerisation reactions at the replication fork of SV40 and, perhaps, also of nuclear chromosomes. DNA polymerase ε, implicated by others as a cell-cycle checkpoint regulator sensing DNA replication lesions, may be dispensable for replication of the small, fast propagating virus that subverts cell cycle controls.
AB - The contributions of DNA polymerases α, δ and ε to SV40 and nuclear DNA syntheses were evaluated. Proteins were UV-crosslinked to nascent DNA within replicating chromosomes and the photolabelled polymerases were immunopurified. Only DNA polymerases α and δ were detectably photolabelled by nascent SV40 DNA, whether synthesized in soluble viral chromatin or within nuclei isolated from SV40-infected cells. In contrast, all three enzymes were photolabelled by the nascent cellular DNA. Mitogenic stimulation enhanced the photolabelling of the polymerases in the α>δ>ε order of preference. The data agree with the notion that DNA polymerases α and δ catalyse the principal DNA polymerisation reactions at the replication fork of SV40 and, perhaps, also of nuclear chromosomes. DNA polymerase ε, implicated by others as a cell-cycle checkpoint regulator sensing DNA replication lesions, may be dispensable for replication of the small, fast propagating virus that subverts cell cycle controls.
KW - Aphidicolin
KW - Butylphenyl-dGTP
KW - Cell cycle checkpoint
KW - DNA polymerases α and δ
KW - Protein-DNA UV-crosslinking
UR - http://www.scopus.com/inward/record.url?scp=0029944855&partnerID=8YFLogxK
U2 - 10.1002/j.1460-2075.1996.tb00583.x
DO - 10.1002/j.1460-2075.1996.tb00583.x
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AN - SCOPUS:0029944855
SN - 0261-4189
VL - 15
SP - 2298
EP - 2305
JO - EMBO Journal
JF - EMBO Journal
IS - 9
ER -