DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation

Katherine Domb, Aviva Katz, Keith D. Harris, Rafael Yaari, Efrat Kaisler, Vu H. Nguyen, Uyen V.T. Hong, Ofir Griess, Karina G. Heskiau, Nir Ohad, Assaf Zemach

Research output: Contribution to journalArticlepeer-review

Abstract

Cytosine (DNA) methylation in plants regulates the expression of genes and transposons. While methylation in plant genomes occurs at CG, CHG, and CHH sequence contexts, the comparative roles of the individual methylation contexts remain elusive. Here, we present Physcomitrella patens as the second plant system, besides Arabidopsis thaliana, with viable mutants with an essentially complete loss of methylation in the CG and non-CG contexts. In contrast to A. thaliana, P. patens has more robust CHH methylation, similar CG and CHG methylation levels, and minimal crosstalk between CG and non-CG methylation, making it possible to study context-specific effects independently. Our data found CHH methylation to act in redundancy with symmetric methylation in silencing transposons and to regulate the expression of CG/CHG-depleted transposons. Specific elimination of CG methylation did not dysregulate transposons or genes. In contrast, exclusive removal of non-CG methylation massively up-regulated transposons and genes. In addition, comparing two exclusively but equally CG- or CHG-methylated genomes, we show that CHG methylation acts as a greater transcriptional regulator than CG methylation. These results disentangle the transcriptional roles of CG and non-CG, as well as symmetric and asymmetric methylation in a plant genome, and point to the crucial role of non-CG methylation in genome regulation.

Original languageEnglish
Pages (from-to)33700-33710
Number of pages11
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number52
DOIs
StatePublished - Dec 2020

Keywords

  • CG methylation
  • CHG methylation
  • CHH methylation
  • Non-CG methylation
  • Transposons

Fingerprint

Dive into the research topics of 'DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation'. Together they form a unique fingerprint.

Cite this