DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli

Ronit Bar Ness-Greenstein; Mel Rosenberg; R. J. Doyle; Nachum Kaplan

doi:10.1016/0378-1097(94)00475-7

DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli

Ronit Bar Ness-Greenstein, Mel Rosenberg^*, R. J. Doyle, Nachum Kaplan

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	125
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(94)00475-7
State	Published - 1 Jan 1995

Keywords

Ammonium sulfate aggregation
Cell surface hydrophobicity
Escherichia coli
Hexadecane
Hydrophobin
Serratia marcescens

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10.1016/0378-1097(94)00475-7

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title = "DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli",

abstract = "In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.",

keywords = "Ammonium sulfate aggregation, Cell surface hydrophobicity, Escherichia coli, Hexadecane, Hydrophobin, Serratia marcescens",

author = "Ness-Greenstein, {Ronit Bar} and Mel Rosenberg and Doyle, {R. J.} and Nachum Kaplan",

note = "Funding Information: We are grateful to Yardena Mazor and Sonia Rosenheck for excellent technical assistance. The work described here was carried out in the Alpha Omega Laboratories of the Maurice and Gabriela Goldschleger School of Dental Medicine, with the support of the United States-Israel Binational Science Foundation, Jerusalem, Israel, Grant 86-0023, and NIH-NIDR Grant DE-07199.",

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AU - Rosenberg, Mel

AU - Doyle, R. J.

AU - Kaplan, Nachum

N1 - Funding Information: We are grateful to Yardena Mazor and Sonia Rosenheck for excellent technical assistance. The work described here was carried out in the Alpha Omega Laboratories of the Maurice and Gabriela Goldschleger School of Dental Medicine, with the support of the United States-Israel Binational Science Foundation, Jerusalem, Israel, Grant 86-0023, and NIH-NIDR Grant DE-07199.

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N2 - In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.

AB - In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.

KW - Ammonium sulfate aggregation

KW - Cell surface hydrophobicity

KW - Escherichia coli

KW - Hexadecane

KW - Hydrophobin

KW - Serratia marcescens

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DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli

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Keywords

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