DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli

Ronit Bar Ness-Greenstein, Mel Rosenberg*, R. J. Doyle, Nachum Kaplan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli, E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalFEMS Microbiology Letters
Volume125
Issue number1
DOIs
StatePublished - 1 Jan 1995

Funding

FundersFunder number
NIH-NIDRDE-07199
National Institute of Dental and Craniofacial ResearchR01DE007199
United States-Israel Binational Science Foundation86-0023

    Keywords

    • Ammonium sulfate aggregation
    • Cell surface hydrophobicity
    • Escherichia coli
    • Hexadecane
    • Hydrophobin
    • Serratia marcescens

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