DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities

Shashank Keshavmurthy; Sung Yin Yang; Ada Alamaru; Yao Yang Chuang; Michel Pichon; David Obura; Silvia Fontana; Stephane De Palmas; Fabrizio Stefani; Francesca Benzoni; Angus MacDonald; Annika M.E. Noreen; Chienshun Chen; Carden C. Wallace; Ruby Moothein Pillay; Vianney Denis; Affendi Yang Amri; James D. Reimer; Takuma Mezaki; Charles Sheppard; Yossi Loya; Avidor Abelson; Mohammed Suleiman Mohammed; Andrew C. Baker; Pargol Ghavam Mostafavi; Budiyanto A. Suharsono; Chaolun Allen Chen

doi:10.1038/srep01520

DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities

Shashank Keshavmurthy, Sung Yin Yang, Ada Alamaru, Yao Yang Chuang, Michel Pichon, David Obura, Silvia Fontana, Stephane De Palmas, Fabrizio Stefani, Francesca Benzoni, Angus MacDonald, Annika M.E. Noreen, Chienshun Chen, Carden C. Wallace, Ruby Moothein Pillay, Vianney Denis, Affendi Yang Amri, James D. Reimer, Takuma Mezaki, Charles SheppardYossi Loya, Avidor Abelson, Mohammed Suleiman Mohammed, Andrew C. Baker, Pargol Ghavam Mostafavi, Budiyanto A. Suharsono, Chaolun Allen Chen

School of Zoology

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Abstract

Stylophora pistillata is a widely used coral "lab-rat" species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacific-Western Australia, Chagos-Madagascar-South Africa, Gulf of Aden-Zanzibar-Madagascar, and Red Sea-Persian/Arabian Gulf-Kenya. On the basis of the fossil record of Stylophora, these four clades diverged from one another 51.5-29.6 Mya, i.e., long before the closure of the Tethyan connection between the tropical Indo-West Pacific and Atlantic in the early Miocene (16-24a Mya) and should be recognised as four distinct species. These findings have implications for comparative ecological and/or physiological studies carried out using Stylophora pistillata as a model species, and highlight the fact that phenotypic plasticity, thought to be common in scleractinian corals, can mask significant genetic variation.

Original language	English
Article number	1520
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep01520
State	Published - 2013

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Keshavmurthy, S., Yang, S. Y., Alamaru, A., Chuang, Y. Y., Pichon, M., Obura, D., Fontana, S., De Palmas, S., Stefani, F., Benzoni, F., MacDonald, A., Noreen, A. M. E., Chen, C., Wallace, C. C., Pillay, R. M., Denis, V., Amri, A. Y., Reimer, J. D., Mezaki, T., ... Chen, C. A. (2013). DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities. Scientific Reports, 3, [1520]. https://doi.org/10.1038/srep01520

@article{555cbb1239134c16a7fbaee74d10eff5,

title = "DNA barcoding reveals the coral {"}laboratory-rat{"}, Stylophora pistillata encompasses multiple identities",

abstract = "Stylophora pistillata is a widely used coral {"}lab-rat{"} species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacific-Western Australia, Chagos-Madagascar-South Africa, Gulf of Aden-Zanzibar-Madagascar, and Red Sea-Persian/Arabian Gulf-Kenya. On the basis of the fossil record of Stylophora, these four clades diverged from one another 51.5-29.6 Mya, i.e., long before the closure of the Tethyan connection between the tropical Indo-West Pacific and Atlantic in the early Miocene (16-24a Mya) and should be recognised as four distinct species. These findings have implications for comparative ecological and/or physiological studies carried out using Stylophora pistillata as a model species, and highlight the fact that phenotypic plasticity, thought to be common in scleractinian corals, can mask significant genetic variation.",

author = "Shashank Keshavmurthy and Yang, {Sung Yin} and Ada Alamaru and Chuang, {Yao Yang} and Michel Pichon and David Obura and Silvia Fontana and {De Palmas}, Stephane and Fabrizio Stefani and Francesca Benzoni and Angus MacDonald and Noreen, {Annika M.E.} and Chienshun Chen and Wallace, {Carden C.} and Pillay, {Ruby Moothein} and Vianney Denis and Amri, {Affendi Yang} and Reimer, {James D.} and Takuma Mezaki and Charles Sheppard and Yossi Loya and Avidor Abelson and Mohammed, {Mohammed Suleiman} and Baker, {Andrew C.} and Mostafavi, {Pargol Ghavam} and Suharsono, {Budiyanto A.} and Chen, {Chaolun Allen}",

note = "Funding Information: The authors wish to thank members of the Coral Reef Evolutionary Ecology and Genetics Laboratory (CREEG), Biodiversity Research Center, Academia Sinica (BRCAS), for assistance with sampling and field logistics (Japan, Taiwan, and Malaysia). S.K. was supported by the national science council (NSC) and Academia Sinica postdoctoral fellowship (2008–2012). The study was supported by grants from the NSC and Academia Sinica (2005–2011) to C.A.C. Thanks to Biological Institute on Kuroshio (Kochi, Japan). FB thanks C. Payri, J-L. Menou, J. Butscher, and the Service Plong{\'e} of IRD Noum{\'e}a for allowing sampling in New Caledonia and Sampling in Djibouti and Saint Brandon{\textquoteright}s was possible thanks to the Tara Oceans Expedition, and to the OCEANS Consortium and to E. Karsenti, E. Bourgois, R. Trouble, and S. Kandels-Lewis. AB thanks Anthony Rouphael and the Saudi Arabian National Commission for Wildlife Conservation and Development (Saudi Arabia); Cathie Page (Lizard Island, Australia); Tara Expeditions (Djibouti); Rachel Silverstein and Adrienne Correa (Western Australia); Akiyuki Irikawa and Ranjeet Bhagooli (Japan); and Tim McClanahan (Kenya). Funding was provided by a grant from the U.S. National Science Foundation (OCE-0099301), a Doctoral Fellowship from the Australian Museum, the Tiffany & Co. Foundation, and the Lenfest Ocean Program. All coral samples were collected with the correct permits. This is the CREEG, BRCAS contribution no. 80.",

year = "2013",

doi = "10.1038/srep01520",

language = "אנגלית",

volume = "3",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

Keshavmurthy, S, Yang, SY, Alamaru, A, Chuang, YY, Pichon, M, Obura, D, Fontana, S, De Palmas, S, Stefani, F, Benzoni, F, MacDonald, A, Noreen, AME, Chen, C, Wallace, CC, Pillay, RM, Denis, V, Amri, AY, Reimer, JD, Mezaki, T, Sheppard, C, Loya, Y , Abelson, A, Mohammed, MS, Baker, AC, Mostafavi, PG, Suharsono, BA & Chen, CA 2013, 'DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities', Scientific Reports, vol. 3, 1520. https://doi.org/10.1038/srep01520

TY - JOUR

T1 - DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities

AU - Keshavmurthy, Shashank

AU - Yang, Sung Yin

AU - Alamaru, Ada

AU - Chuang, Yao Yang

AU - Pichon, Michel

AU - Obura, David

AU - Fontana, Silvia

AU - De Palmas, Stephane

AU - Stefani, Fabrizio

AU - Benzoni, Francesca

AU - MacDonald, Angus

AU - Noreen, Annika M.E.

AU - Chen, Chienshun

AU - Wallace, Carden C.

AU - Pillay, Ruby Moothein

AU - Denis, Vianney

AU - Amri, Affendi Yang

AU - Reimer, James D.

AU - Mezaki, Takuma

AU - Sheppard, Charles

AU - Loya, Yossi

AU - Abelson, Avidor

AU - Mohammed, Mohammed Suleiman

AU - Baker, Andrew C.

AU - Mostafavi, Pargol Ghavam

AU - Suharsono, Budiyanto A.

AU - Chen, Chaolun Allen

N1 - Funding Information: The authors wish to thank members of the Coral Reef Evolutionary Ecology and Genetics Laboratory (CREEG), Biodiversity Research Center, Academia Sinica (BRCAS), for assistance with sampling and field logistics (Japan, Taiwan, and Malaysia). S.K. was supported by the national science council (NSC) and Academia Sinica postdoctoral fellowship (2008–2012). The study was supported by grants from the NSC and Academia Sinica (2005–2011) to C.A.C. Thanks to Biological Institute on Kuroshio (Kochi, Japan). FB thanks C. Payri, J-L. Menou, J. Butscher, and the Service Plongé of IRD Nouméa for allowing sampling in New Caledonia and Sampling in Djibouti and Saint Brandon’s was possible thanks to the Tara Oceans Expedition, and to the OCEANS Consortium and to E. Karsenti, E. Bourgois, R. Trouble, and S. Kandels-Lewis. AB thanks Anthony Rouphael and the Saudi Arabian National Commission for Wildlife Conservation and Development (Saudi Arabia); Cathie Page (Lizard Island, Australia); Tara Expeditions (Djibouti); Rachel Silverstein and Adrienne Correa (Western Australia); Akiyuki Irikawa and Ranjeet Bhagooli (Japan); and Tim McClanahan (Kenya). Funding was provided by a grant from the U.S. National Science Foundation (OCE-0099301), a Doctoral Fellowship from the Australian Museum, the Tiffany & Co. Foundation, and the Lenfest Ocean Program. All coral samples were collected with the correct permits. This is the CREEG, BRCAS contribution no. 80.

PY - 2013

Y1 - 2013

N2 - Stylophora pistillata is a widely used coral "lab-rat" species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacific-Western Australia, Chagos-Madagascar-South Africa, Gulf of Aden-Zanzibar-Madagascar, and Red Sea-Persian/Arabian Gulf-Kenya. On the basis of the fossil record of Stylophora, these four clades diverged from one another 51.5-29.6 Mya, i.e., long before the closure of the Tethyan connection between the tropical Indo-West Pacific and Atlantic in the early Miocene (16-24a Mya) and should be recognised as four distinct species. These findings have implications for comparative ecological and/or physiological studies carried out using Stylophora pistillata as a model species, and highlight the fact that phenotypic plasticity, thought to be common in scleractinian corals, can mask significant genetic variation.

AB - Stylophora pistillata is a widely used coral "lab-rat" species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacific-Western Australia, Chagos-Madagascar-South Africa, Gulf of Aden-Zanzibar-Madagascar, and Red Sea-Persian/Arabian Gulf-Kenya. On the basis of the fossil record of Stylophora, these four clades diverged from one another 51.5-29.6 Mya, i.e., long before the closure of the Tethyan connection between the tropical Indo-West Pacific and Atlantic in the early Miocene (16-24a Mya) and should be recognised as four distinct species. These findings have implications for comparative ecological and/or physiological studies carried out using Stylophora pistillata as a model species, and highlight the fact that phenotypic plasticity, thought to be common in scleractinian corals, can mask significant genetic variation.

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DO - 10.1038/srep01520

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VL - 3

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 1520

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DNA barcoding reveals the coral "laboratory-rat", Stylophora pistillata encompasses multiple identities

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