Distinguishing between retention signals and degrons acting in ERAD

Ilana Shapira, Dana Charuvi, Yechiel Elkabetz, Koret Hirschberg, Shoshana Bar-Nun

Research output: Contribution to journalArticlepeer-review

Abstract

Endoplasmic reticulum-associated degradation (ERAD) eliminates aberrant proteins from the secretory pathway. Such proteins are retained in the endoplasmic reticulum and targeted for degradation by the ubiquitin-proteasome system. Cis-acting motifs can function in ERAD as retention signals, preventing vesicular export from the endoplasmic reticulum, or as degrons, targeting proteins for degradation. Here, we show that μstp, the C-terminal 20-residue tailpiece of the secretory IgM μs heavy chain, functions both as a portable retention signal and as an ERAD degron. Retention of μstp fusions of secreted versions of thyroid peroxidase and yellow fluorescent protein in the endoplasmic reticulum requires the presence of the penultimate cysteine of μstp. In its role as a portable degron, the μstp targets the retained proteins for ERAD but does not serve as an obligatory ubiquitin-conjugation site. Abolishing μstp glycosylation accelerates the degradation of both μstpCys-fused substrates, yet absence of the N-glycan eliminates the requirement for the penultimate cysteine in the retention and degradation of the unglycosylated yellow fluorescent protein. Hence, the dual role played by the μstpCys motif as a retention signal and as a degron can be attributed to distinct elements within this sequence.

Original languageEnglish
Pages (from-to)4377-4387
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number24
DOIs
StatePublished - 15 Dec 2007

Keywords

  • Degrons
  • ERAD
  • Proteasome
  • Retention signals

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