Distinct spatiotemporal features of microglia and monocyte-derived macrophages in glioma

Kaveri Banerjee, Avinoam Ratzabi, Itai M. Caspit, Or Ganon, Pablo Blinder, Steffen Jung, Reuven Stein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Gliomas are the most frequent primary tumors of the brain. Glioma progression is regulated by the tumor microenvironment, which is mainly composed of tumor-associated microglia (TA-MG) and monocyte-derived macrophages (MDM). Recent studies have highlighted the distinct properties of these cells in glioma progression. However, their spatiotemporal alteration during tumor progression has not been fully explored. Using a genetic lineage tracing approach, we show that TA-MG and MDMs differ in their spatiotemporal distribution and interaction with other components of the glioma microenvironment. MDM were present only inside the tumor, whereas TA-MG accumulated both outside and inside the tumor. However, TA-MG was eliminated from the tumor mass as the tumor progressed. Depletion of MDM led to enhanced occupancy of TA-MG in the tumor core, indicating that TA-MG elimination was regulated by MDM. TA-MG and MDM are heterogeneous cell populations whose compositions and properties can change during tumor progression. Finally, MG, TA-MG and MDM were enriched in the perivascular area (PVA) compared to more distal blood vessel-associated areas. However, inside the tumor, the MDM enrichment in PVA was higher than that in TA-MG. Collectively, we established that TA-MG and MDM exhibit different spatiotemporal features in glioma, suggesting distinctive roles during tumor progression.

Original languageEnglish
Article number2250161
JournalEuropean Journal of Immunology
Issue number4
StatePublished - Apr 2023


  • glioma
  • microglia
  • monocyte-derived macrophages
  • tumor microenvironment


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