Disruption of the Aspergillus fumigatus ECM33 homologue results in rapid conidial germination, antifungal resistance and hypervirulence

Jacob Romano, Guy Nimrod, Nir Ben-Tal, Yona Shadkchan, Koti Baruch, Haim Sharon, Nir Osherov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The ECM33/SPS2 family of glycosylphosphatidylinositol-anchored proteins plays an important role in maintaining fungal cell wall integrity and virulence. However, the precise molecular role of these proteins is unknown. In this work, AfuEcm33, the gene encoding the ECM33 homologue in the important pathogenic fungus Aspergillus fumigatus, has been cloned and its function analysed. It is shown that disruption of AfuEcm33 results in rapid conidial germination, increased cell-cell adhesion, resistance to the antifungal agent caspofungin and increased virulence in an immunocompromised mouse model for disseminated aspergillosis. These results suggest that the protein encoded by AfuEcm33 is involved in key aspects of cell wall morphogenesis and plays an important role in A. fumigatus virulence.

Original languageEnglish
Pages (from-to)1919-1928
Number of pages10
JournalMicrobiology
Volume152
Issue number7
DOIs
StatePublished - Jul 2006

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