@article{00eaa2e5b6324cf0bc044792c6120f3b,
title = "Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules",
abstract = "Many important proteins undergo pH-dependent conformational changes resulting in “on-off” switches for protein function, which are essential for regulation of life processes and have wide application potential. Here, we report a pair of cellulosomal assembly modules, comprising a cohesin and a dockerin from Clostridium acetobutylicum, which interact together following a unique pH-dependent switch between two functional sites rather than on-off states. The two cohesin-binding sites on the dockerin are switched from one to the other at pH 4.8 and 7.5 with a 180° rotation of the bound dockerin. Combined analysis by nuclear magnetic resonance spectroscopy, crystal structure determination, mutagenesis, and isothermal titration calorimetry elucidates the chemical and structural mechanism of the pH-dependent switching of the binding sites. The pH-dependent dual-binding-site switch not only represents an elegant example of biological regulation but also provides a new approach for developing pH-dependent protein devices and biomaterials beyond an on-off switch for biotechnological applications.",
author = "Xingzhe Yao and Chao Chen and Yefei Wang and Sheng Dong and Liu, {Ya Jun} and Yifei Li and Zhenling Cui and Weibin Gong and Sarah Perrett and Lishan Yao and Raphael Lamed and Bayer, {Edward A.} and Qiu Cui and Yingang Feng",
note = "Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",
year = "2020",
month = oct,
day = "23",
doi = "10.1126/sciadv.abd7182",
language = "אנגלית",
volume = "6",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "43",
}