Directed metallization of single-enzyme molecules with preserved enzymatic activity

Sefi Vernick*, Hila Moscovich-Dagan, Carmit Porat-Ophir, Judith Rishpon, Amihay Freeman, Yosi Shacham-Diamand

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A new method for the fabrication of molecular, water-soluble, and biologically active enzymemetal hybrids was designed and its feasibility demonstrated. The method is based on the display of nucleation sites directing a subsequent electroless deposition of palladium and other metals to the enzyme's surface. The process is carried out under mild physiological conditions, enabling the preservation of enzymatic activity and water solubility. The feasibility of the new method was demonstrated by using the enzyme glucose oxidase and palladium combination as the first model system. The glucose oxidasepalladium hybrid thus obtained retained their solubility and enzymatic glucose oxidation capabilities. Hybrids immobilized on platinum electrodes exhibited nanowiring and effective direct electron transfer from the enzyme catalytic site to the electrode. The new enzymemetal hybrids thus obtained may be readily incorporated into miniaturic biosensors and biochips, used as novel antibacterial agents or as markers for improved invivo imaging. Furthermore, the methodology developed may be readily extended to a series of metal coatings on the surface of biologically active proteins.

Original languageEnglish
Article number4625952
Pages (from-to)95-99
Number of pages5
JournalIEEE Transactions on Nanotechnology
Issue number1
StatePublished - Jan 2009


  • Biosensors
  • Electroless deposition
  • Metal-enzyme hybrid
  • Metallization


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