Direct ultrasound application had no effect on cardiac hemodynamic performance in a baseline isolated rat heart model

Shai Greenberg, Ariel Finkelstein, Ela Raisman, Itzhak Shapira, Ran Koronowski, Shlomo Laniado, Gad Keren

Research output: Contribution to journalArticlepeer-review


Therapeutic ultrasound (US) has been used for more than 3 decades to promote tissue healing in cases of tissue injury and muscle soreness. It was previously suggested that US may have vasorelaxatory and inotropic properties. However, the direct effect of therapeutic US in a whole heart model has not yet been investigated. Our hypothesis was that application of US might enhance cardiac function. The Langendort model was modified in a special manner to allow application of US to the heart. Using this model, 20 male rats were equally divided into two groups. Group 1: the hearts were perfused for 15 min, to obtain baseline measurements, and then they were perfused for another 15 min in a special bath full of perfusate. Group 2: after 15 min of baseline measurements, continuous US of 1 MHz 2 W/cm2 was applied for another 15 min. The parameters that were measured at 5-min intervals were: left ventricular pressure P(max), first derivative of the rise and fall in left ventricular pressure (dP/dtmax, dP/dtmin), and pressure-time integral. There was no significant difference between the two groups in all parameters at baseline and during US application. P(max) and dP/dtmax remained constant. After 15 min of US propagation, P(max) was 98% ± 3 from baseline level vs. 98% ± 7 in the control group, and dP/dtmax was 98% ± 3 vs. 99% ± 9 in the control. In dP/dtmin, a gradual decline after 15 min of perfusion was measured. In the US- treated group, it declined to 80% ± 10 vs. 83% ± 5 in the controls. In conclusion, US radiation at the dose specified does not improve healthy isolated heart hemodynamic performance. We established a model that may be used for further investigation.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalUltrasound in Medicine and Biology
Issue number2
StatePublished - Feb 2000
Externally publishedYes


  • Heart muscle
  • Isolated heart
  • Ultrasound


Dive into the research topics of 'Direct ultrasound application had no effect on cardiac hemodynamic performance in a baseline isolated rat heart model'. Together they form a unique fingerprint.

Cite this