Direct contact test for evaluating bacterial growth on machined and rough surface implants: An in vitro study

Shimshon Slutzkey*, Ofer Moses, Haim Tal, Avi Meirowitz, Shlomo Matalon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Objective: To use a modified direct contact test (DCT) to evaluate the bacterial growth on 3 commercially available implants. Materials and Methods: Streptococcus salivarius growth was tested on 1 machined surface and 2 commercial rough surface implants. Seven implants from each group were fixed to the cover of a 96-well microtiter plate. Bacterial suspension was placed on each implant, and the plate was incubated at 37°C for 1 hour to allow the suspension fluid to evaporate. Fresh medium was added to 7 corresponding wells in the microtiter plate, and the cover holding the implants was placed on the plate to enable immersion of the implant. Noncontaminated implant samples served as control. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 hours. Results: There was a significant S. salivarius growth in all groups of test implants compared with the control. There was no significant difference between bacterial growth kinetics on treated and nontreated implant surfaces (P = 0.241). Conclusion: Using the DCT, we found no difference in bacterial growth between machined and rough surface implants.

Original languageEnglish
Pages (from-to)899-902
Number of pages4
JournalImplant Dentistry
Volume26
Issue number6
DOIs
StatePublished - Dec 2017

Funding

FundersFunder number
Ernst and Tova Turnheim

    Keywords

    • Dental
    • Kinetics
    • Microbiota
    • Titanium

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