TY - JOUR
T1 - DIP and DIP + 2 as glutathione oxidants and radiation sensitizers in cultured chinese hamster cells
AU - Harris, J. W.
AU - Power, J. A.
AU - Kosower, N. S.
AU - Kosower, E. M.
N1 - Funding Information:
This work was supported by the U .S . Energy Research and Development Administration . The participation of NSK was made possible by support from the Chief Scientist's Office, Ministry of Health, Israel, and of EMK by support from the Petroleum Research Fund, American Chemical Society .
PY - 1975
Y1 - 1975
N2 - Two diamide analogues, diazene dicarboxylic acid bis (N'methylpiperazide) or DIP, and its bis-N'methyl iodide salt, or DIP + 2, were tested for their ability to penetrate cultured Chinese hamster cells and oxidize intracellular glutathione. DIP penetrated the cells at a reasonable rate at 18°C, 160 nmoles being required to oxidize the endogenous glutathione of 2 × 106 cells, but it penetrated very slowly at 0°C. DIP + 2 did not effectively oxidize glutathione in Chinese hamster cells, possibly because it did not enter the cells. DIP became toxic after about 10 min of exposure, but its toxicity could be moderated by using anoxic conditions. DIP, but not DIP + 2, sensitized anoxic Chinese hamster cells to X-radiation by a factor of 1·5, an effect that was due entirely to removal of the shoulder from the survival curve.
AB - Two diamide analogues, diazene dicarboxylic acid bis (N'methylpiperazide) or DIP, and its bis-N'methyl iodide salt, or DIP + 2, were tested for their ability to penetrate cultured Chinese hamster cells and oxidize intracellular glutathione. DIP penetrated the cells at a reasonable rate at 18°C, 160 nmoles being required to oxidize the endogenous glutathione of 2 × 106 cells, but it penetrated very slowly at 0°C. DIP + 2 did not effectively oxidize glutathione in Chinese hamster cells, possibly because it did not enter the cells. DIP became toxic after about 10 min of exposure, but its toxicity could be moderated by using anoxic conditions. DIP, but not DIP + 2, sensitized anoxic Chinese hamster cells to X-radiation by a factor of 1·5, an effect that was due entirely to removal of the shoulder from the survival curve.
UR - http://www.scopus.com/inward/record.url?scp=0016629179&partnerID=8YFLogxK
U2 - 10.1080/09553007514551261
DO - 10.1080/09553007514551261
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AN - SCOPUS:0016629179
SN - 0955-3002
VL - 28
SP - 439
EP - 445
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 5
ER -