TY - JOUR
T1 - Digoxin-Like Immunoreactivity
T2 - Occurrence in Three Molecular Forms and Partial Characterization
AU - Shilo, L.
AU - Werber, M. M.
AU - Dolev, S.
AU - Shapiro, M.
AU - Shenkman, L.
PY - 1987/6
Y1 - 1987/6
N2 - Digoxin-like immunoreactivity (DLI) has been found in serum from subjects with a variety of physiological and pathological conditions, including uremia, liver disease, pregnancy, and the neonatal period. The physicochemical nature of this material is still not known. Using gel filtration chromatography, extraction with methylene chloride, and treatment with β-glucuronidase, we determined that serum DLI exists in three forms: protein-bound, glucuronidated, and free, with respective mol wt of 5000, 400, and 230. In serum, DLI exists in the proteinbound and free forms, while in urine, DLI is found in the glucuronidated and free states.
AB - Digoxin-like immunoreactivity (DLI) has been found in serum from subjects with a variety of physiological and pathological conditions, including uremia, liver disease, pregnancy, and the neonatal period. The physicochemical nature of this material is still not known. Using gel filtration chromatography, extraction with methylene chloride, and treatment with β-glucuronidase, we determined that serum DLI exists in three forms: protein-bound, glucuronidated, and free, with respective mol wt of 5000, 400, and 230. In serum, DLI exists in the proteinbound and free forms, while in urine, DLI is found in the glucuronidated and free states.
UR - http://www.scopus.com/inward/record.url?scp=0023244936&partnerID=8YFLogxK
U2 - 10.1210/jcem-64-6-1257
DO - 10.1210/jcem-64-6-1257
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AN - SCOPUS:0023244936
SN - 0021-972X
VL - 64
SP - 1257
EP - 1260
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 6
ER -