Digoxin-Like Immunoreactivity: Occurrence in Three Molecular Forms and Partial Characterization

L. Shilo, M. M. Werber, S. Dolev, M. Shapiro, L. Shenkman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Digoxin-like immunoreactivity (DLI) has been found in serum from subjects with a variety of physiological and pathological conditions, including uremia, liver disease, pregnancy, and the neonatal period. The physicochemical nature of this material is still not known. Using gel filtration chromatography, extraction with methylene chloride, and treatment with β-glucuronidase, we determined that serum DLI exists in three forms: protein-bound, glucuronidated, and free, with respective mol wt of 5000, 400, and 230. In serum, DLI exists in the proteinbound and free forms, while in urine, DLI is found in the glucuronidated and free states.

Original languageEnglish
Pages (from-to)1257-1260
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume64
Issue number6
DOIs
StatePublished - Jun 1987

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