TY - JOUR
T1 - Differing proliferative responses in proximal and distal colons of growing rats fed food eaten by adenoma patients
AU - Liberman, Vivian
AU - Nyska, Abraham
AU - Kashtan, Hanoch
AU - Zajicek, Gershom
AU - Lubin, Flora
AU - Rozen, Paul
PY - 1996
Y1 - 1996
N2 - Animal dietary studies related to human colorectal carcinogenesis are usually based on AIN-76A diet, which is dissimilar to human food in source, preparation, and content. Our aim was to examine colonic epithelial proliferation in rats fed a diet based on the mean daily food intake of adenoma patients. Foods were prepared as reported by the adenoma patients and dehydrated; 64 Sprague-Dawley rats were fed either 'human adenoma' or AIN-76A diet and every eight weeks, eight from each group were sacrificed. Both groups gained weight equally, had no colonic histological changes, but during the study showed progressive lengthening of colonic crypts (P < 0.01) and decreased proliferation (P < 0.05) in distal colons. Compared to controls, rats fed human adenoma diet had significantly longer crypts (P < 0.01) and more labeled cells (P < 0.05) at 32 weeks; overall they had increased proliferation (P < 0.01), most significantly in the distal colon. Thus, food eaten by adenoma patients induced hyperproliferative changes in the rat colon during growth and maturity, especially the distal colon, as found in humans at risk for neoplasia.
AB - Animal dietary studies related to human colorectal carcinogenesis are usually based on AIN-76A diet, which is dissimilar to human food in source, preparation, and content. Our aim was to examine colonic epithelial proliferation in rats fed a diet based on the mean daily food intake of adenoma patients. Foods were prepared as reported by the adenoma patients and dehydrated; 64 Sprague-Dawley rats were fed either 'human adenoma' or AIN-76A diet and every eight weeks, eight from each group were sacrificed. Both groups gained weight equally, had no colonic histological changes, but during the study showed progressive lengthening of colonic crypts (P < 0.01) and decreased proliferation (P < 0.05) in distal colons. Compared to controls, rats fed human adenoma diet had significantly longer crypts (P < 0.01) and more labeled cells (P < 0.05) at 32 weeks; overall they had increased proliferation (P < 0.01), most significantly in the distal colon. Thus, food eaten by adenoma patients induced hyperproliferative changes in the rat colon during growth and maturity, especially the distal colon, as found in humans at risk for neoplasia.
KW - Animal model
KW - Colorectal cancer
KW - Diet
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=0029891110&partnerID=8YFLogxK
U2 - 10.1007/BF02088219
DO - 10.1007/BF02088219
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C2 - 8654134
AN - SCOPUS:0029891110
SN - 0163-2116
VL - 41
SP - 1057
EP - 1064
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 6
ER -