Differentiation of 23-S ribosomal RNA cistrons from 16-S ribosomal RNA cistrons in Escherichia coli by the sequence complementarity test

Esther Segal*, Moshe Osenholts, Saul A. Yankofsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

It has been reported that the 16-S and 23-S components of Escherichia coli ribosomal RNA compete for common hybridization sites on homologous DNA. We here show that no such competition phenomenon occurs. Carefully purified preparations of the two major ribosomal RNA types do not competitively interact with one another during hybridization to Escherichia coli DNA. Consequently, as in all other organisms tested to date, genes coding for molecules of 23-S ribosomal RNA are entirely distinct from those giving rise to 16-S ribosomal RNA molecules. We also confirm that there are multiple gene copies responsible for the biogenesis of each ribosomal RNA component in Escherichia coli.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume308
Issue number1
DOIs
StatePublished - 21 Apr 1973

Funding

FundersFunder number
Tel Aviv University

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