TY - JOUR
T1 - Differentiation of 23-S ribosomal RNA cistrons from 16-S ribosomal RNA cistrons in Escherichia coli by the sequence complementarity test
AU - Segal, Esther
AU - Osenholts, Moshe
AU - Yankofsky, Saul A.
N1 - Funding Information:
We are indebted to Dr Eugene Rosenberg for his constructive criticism during the preparation of this manuscript. This work was entirely supported by departmental research funds of Tel Aviv University.
PY - 1973/4/21
Y1 - 1973/4/21
N2 - It has been reported that the 16-S and 23-S components of Escherichia coli ribosomal RNA compete for common hybridization sites on homologous DNA. We here show that no such competition phenomenon occurs. Carefully purified preparations of the two major ribosomal RNA types do not competitively interact with one another during hybridization to Escherichia coli DNA. Consequently, as in all other organisms tested to date, genes coding for molecules of 23-S ribosomal RNA are entirely distinct from those giving rise to 16-S ribosomal RNA molecules. We also confirm that there are multiple gene copies responsible for the biogenesis of each ribosomal RNA component in Escherichia coli.
AB - It has been reported that the 16-S and 23-S components of Escherichia coli ribosomal RNA compete for common hybridization sites on homologous DNA. We here show that no such competition phenomenon occurs. Carefully purified preparations of the two major ribosomal RNA types do not competitively interact with one another during hybridization to Escherichia coli DNA. Consequently, as in all other organisms tested to date, genes coding for molecules of 23-S ribosomal RNA are entirely distinct from those giving rise to 16-S ribosomal RNA molecules. We also confirm that there are multiple gene copies responsible for the biogenesis of each ribosomal RNA component in Escherichia coli.
UR - http://www.scopus.com/inward/record.url?scp=0015931820&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(73)90124-X
DO - 10.1016/0005-2787(73)90124-X
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AN - SCOPUS:0015931820
SN - 0005-2787
VL - 308
SP - 79
EP - 87
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -