Differentiation-associated changes of cation-transport activities in myeloid leukemic cell lines

Ada Rephaeli, Adina Aviram, Talma Englender, Mati Shaklai

Research output: Contribution to journalArticlepeer-review


Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5-10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluorescent probe, fura-2 acetoxymethyl ester. The increased 45Ca2+ uptake was accompanied by a decrease in ouabain-insensitive and -sensitive 86Rb+ uptake. Furthermore, correlation between the changes in these activities was observed. Modulation of extracellular pH affected differentiation: higher pH increased the extent of differentiation.

Original languageEnglish
Pages (from-to)401-405
Number of pages5
JournalFEBS Letters
Issue number2
StatePublished - 29 Aug 1988


  • (HL-60 leukemic cell, U-937 leukemic cell)
  • Ca
  • Rb transport
  • Retinoic acid


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