Differentiation and characterization of Tr1 cells

Norio Chihara, Asaf Madi, Katarzyna Karwacz, Amit Awasthi, Vijay K. Kuchroo

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Regulatory T cell-mediated suppression serves as a pivotal mechanism of negative regulation of immune-mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that prevent excessive inflammatory responses and maintain immune tolerance. The antiinflammatory role of Tr1 cells is mediated in part by their production of interleukin 10 (IL-10), which dampens the function of both antigen-presenting cells and antigen-specific effector T cells. Additionally, Tr1 cells can kill effector and myeloid cells through the perforin-granzyme B pathway. Adoptive transfer of in vitro differentiated Tr1 cells can be used to suppress autoimmune tissue inflammation in vivo. This unit describes the in vitro stimulation of naïve murine CD4+ T cells using IL-27 to generate IL-10-producing Tr1 cells.

Original languageEnglish
Pages (from-to)3.27.1-3.27.10
JournalCurrent Protocols in Immunology
Volume2016
DOIs
StatePublished - 2016
Externally publishedYes

Funding

FundersFunder number
National Institutes of HealthNS076410, NS045937
National Institute of Allergy and Infectious DiseasesP01AI073748
Japan Society for the Promotion of Science
The Wellcome Trust DBT India Alliance

    Keywords

    • CD4
    • IL-10
    • IL-27
    • T cell differentiation
    • Tr1

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