Differential sensitivity of MCF-7 and LCC2 cells, to multiple growth inhibitory agents: Possible relation to high bcl-2/bax ratio?

G. Lilling; H. Hacohen; J. Nordenberg; T. Livnat; V. Rotter; Y. Sidi

doi:10.1016/S0304-3835(00)00579-6

Differential sensitivity of MCF-7 and LCC2 cells, to multiple growth inhibitory agents: Possible relation to high bcl-2/bax ratio?

G. Lilling, H. Hacohen, J. Nordenberg, T. Livnat, V. Rotter, Y. Sidi^*

^*Corresponding author for this work

Hematology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Comparison of LCC2, the E₂-independent, tamoxifen-resistant subline of the MCF-7 human breast cancer cell line with its parent line, disclosed that it is more resistant to growth inhibition and apoptosis induction by a variety of agents acting by diverse mechanisms. Thus, LCC2 cells can serve as a useful in-vitro model for the study of the molecular mechanisms of this resistance. It was found that bcl-2 protein and mRNA were elevated and that bax protein and mRNA were reduced in LCC2 compared with MCF-7 cells. Incubation of both lines in the presence of bcl-2 antisense caused growth inhibition and reduced bcl-2 protein levels only in MCF-7 cells, suggesting the involvement of bcl-2 in the regulation of normal growth of breast cancer cells. Increased bcl-2 expression in breast cancer cells may correlate with their resistance to growth inhibitory agents. Bcl-2 is a useful target for enhancing the effects of growth inhibitory agents. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	27-34
Number of pages	8
Journal	Cancer Letters
Volume	161
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(00)00579-6
State	Published - 8 Dec 2000

Funding

Funders	Funder number
Tel Aviv University

Keywords

LCC2 cells
MCF-7 human breast cancer cell line
Sensitivity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(00)00579-6

Cite this

@article{0220bbb19e5d412095fa600ba1168b80,

title = "Differential sensitivity of MCF-7 and LCC2 cells, to multiple growth inhibitory agents: Possible relation to high bcl-2/bax ratio?",

abstract = "Comparison of LCC2, the E2-independent, tamoxifen-resistant subline of the MCF-7 human breast cancer cell line with its parent line, disclosed that it is more resistant to growth inhibition and apoptosis induction by a variety of agents acting by diverse mechanisms. Thus, LCC2 cells can serve as a useful in-vitro model for the study of the molecular mechanisms of this resistance. It was found that bcl-2 protein and mRNA were elevated and that bax protein and mRNA were reduced in LCC2 compared with MCF-7 cells. Incubation of both lines in the presence of bcl-2 antisense caused growth inhibition and reduced bcl-2 protein levels only in MCF-7 cells, suggesting the involvement of bcl-2 in the regulation of normal growth of breast cancer cells. Increased bcl-2 expression in breast cancer cells may correlate with their resistance to growth inhibitory agents. Bcl-2 is a useful target for enhancing the effects of growth inhibitory agents. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "LCC2 cells, MCF-7 human breast cancer cell line, Sensitivity",

author = "G. Lilling and H. Hacohen and J. Nordenberg and T. Livnat and V. Rotter and Y. Sidi",

note = "Funding Information: The research was supported by the chief scientist of the Israeli ministry of health and by the Hamer grant of the Tel Aviv University medical school. The authors wish to thank Dr Robert Clarke from the Lombardi Cancer Research Center, Georgetown University Medical Center for providing us the MCF-7 and LCC2 cells.",

year = "2000",

month = dec,

day = "8",

doi = "10.1016/S0304-3835(00)00579-6",

language = "אנגלית",

volume = "161",

pages = "27--34",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Differential sensitivity of MCF-7 and LCC2 cells, to multiple growth inhibitory agents

T2 - Possible relation to high bcl-2/bax ratio?

AU - Lilling, G.

AU - Hacohen, H.

AU - Nordenberg, J.

AU - Livnat, T.

AU - Rotter, V.

AU - Sidi, Y.

N1 - Funding Information: The research was supported by the chief scientist of the Israeli ministry of health and by the Hamer grant of the Tel Aviv University medical school. The authors wish to thank Dr Robert Clarke from the Lombardi Cancer Research Center, Georgetown University Medical Center for providing us the MCF-7 and LCC2 cells.

PY - 2000/12/8

Y1 - 2000/12/8

N2 - Comparison of LCC2, the E2-independent, tamoxifen-resistant subline of the MCF-7 human breast cancer cell line with its parent line, disclosed that it is more resistant to growth inhibition and apoptosis induction by a variety of agents acting by diverse mechanisms. Thus, LCC2 cells can serve as a useful in-vitro model for the study of the molecular mechanisms of this resistance. It was found that bcl-2 protein and mRNA were elevated and that bax protein and mRNA were reduced in LCC2 compared with MCF-7 cells. Incubation of both lines in the presence of bcl-2 antisense caused growth inhibition and reduced bcl-2 protein levels only in MCF-7 cells, suggesting the involvement of bcl-2 in the regulation of normal growth of breast cancer cells. Increased bcl-2 expression in breast cancer cells may correlate with their resistance to growth inhibitory agents. Bcl-2 is a useful target for enhancing the effects of growth inhibitory agents. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Comparison of LCC2, the E2-independent, tamoxifen-resistant subline of the MCF-7 human breast cancer cell line with its parent line, disclosed that it is more resistant to growth inhibition and apoptosis induction by a variety of agents acting by diverse mechanisms. Thus, LCC2 cells can serve as a useful in-vitro model for the study of the molecular mechanisms of this resistance. It was found that bcl-2 protein and mRNA were elevated and that bax protein and mRNA were reduced in LCC2 compared with MCF-7 cells. Incubation of both lines in the presence of bcl-2 antisense caused growth inhibition and reduced bcl-2 protein levels only in MCF-7 cells, suggesting the involvement of bcl-2 in the regulation of normal growth of breast cancer cells. Increased bcl-2 expression in breast cancer cells may correlate with their resistance to growth inhibitory agents. Bcl-2 is a useful target for enhancing the effects of growth inhibitory agents. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - LCC2 cells

KW - MCF-7 human breast cancer cell line

KW - Sensitivity

UR - https://www.scopus.com/pages/publications/0034624329

U2 - 10.1016/S0304-3835(00)00579-6

DO - 10.1016/S0304-3835(00)00579-6

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 11078910

AN - SCOPUS:0034624329

SN - 0304-3835

VL - 161

SP - 27

EP - 34

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Differential sensitivity of MCF-7 and LCC2 cells, to multiple growth inhibitory agents: Possible relation to high bcl-2/bax ratio?

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this