Differential regulation of tumour necrosis factor receptors (TNF-R) by IL-4; upregulation of P55 and P75 TNF-R on synovial joint mononuclear cells

Interleukin 4 (IL-4) has previously been shown to downregulate the production of pro-inflammatory cytokines such as TNF-α, and hence has been considered to be a potential anti-inflammatory agent. In this study we have investigated the effects of IL-4 on the expression of both p55 and p75 TNF receptors (TNF-R) by flow cytometry and radioligand binding analyses and demonstrate that IL-4 downregulates both p55 and p75 TNF-R on HeLa and Jijoye cell lines in a dose dependent manner. IL-4 reduced the number of p55 TNF-R on HeLa cells from 6400 (K_d 5.1 nM) to 3900 (K_d 3.7 nM), and p75 TNF-R on Jijoye cells from 4800 (K_d 1.6 nM) to 3250 (K_d 1.5 nM). However, different effects were observed on peripheral blood mononuclear cells (PBMC). IL-4 inhibited the increase in p55 and p75 TNF-R on PBMC following adherence, whereas IL-4 upregulated p75 TNF-R expressed on PHA induced T cell blasts. To assess further the possible anti-inflammatory properties of IL-4, we studied its effects on synovial joint mononuclear cell cultures from 15 patients with inflammatory synovitis. In contrast to the differential effects of IL-4 on monocytes and T cells, IL-4 upregulated both p55 (P < 0.05) and p75 TNF-R (P < 0.005) on synovial joint cells in culture. IL-4 treatment caused a small decrease in levels of bioactive TNF-α in RA synovial culture supernatants, together with an increase in soluble p75 TNF-R levels although differences were not significant. These data demonstrate that IL-4 can modulate both TNF-α and TNF-R expression, and provides further evidence for the role of soluble TNF-R as endogenous regulators of TNF-α function.