TY - JOUR
T1 - Differential regulation of activity-dependent neuroprotective protein in rat astrocytes by VIP and PACAP
AU - Zusev, Margalit
AU - Gozes, Illana
N1 - Funding Information:
This study was supported by ISF, BSF, the Lily and Avraham Gildor Chair for Investigation of Growth Factors, Allon Therapeutics and the Institute for the Study of Aging.
PY - 2004/12/15
Y1 - 2004/12/15
N2 - Activity-dependent neuroprotective protein (ADNP) was shown to be a vasoactive intestinal peptide (VIP) responsive gene in astrocytes derived from the cerebral cortex of newborn rats. The present study was set out to identify VIP receptors that are associated with increases in ADNP expression in developing astrocytes. Using VIP analogues specific for the VPAC1 and the VPAC2 receptors, it was discovered that VIP induced changes in ADNP expression in astrocytes via the VPAC2 receptor. The constitutive synthesis of ADNP and VPAC2 was shown to be age-dependent and increased as the astrocyte culture developed. Pituitary adenylate cyclase-activating polypeptide (PACAP) also induced changes in ADNP expression. The apparent changes induced by VIP and PACAP on ADNP expression were developmentally dependent, and while stimulating expression in young astrocytes, an inhibition was demonstrated in older cultures. In conclusion, VIP, PACAP and the VPAC2 receptor may all contribute to the regulation of ADNP gene expression in the developing astrocyte.
AB - Activity-dependent neuroprotective protein (ADNP) was shown to be a vasoactive intestinal peptide (VIP) responsive gene in astrocytes derived from the cerebral cortex of newborn rats. The present study was set out to identify VIP receptors that are associated with increases in ADNP expression in developing astrocytes. Using VIP analogues specific for the VPAC1 and the VPAC2 receptors, it was discovered that VIP induced changes in ADNP expression in astrocytes via the VPAC2 receptor. The constitutive synthesis of ADNP and VPAC2 was shown to be age-dependent and increased as the astrocyte culture developed. Pituitary adenylate cyclase-activating polypeptide (PACAP) also induced changes in ADNP expression. The apparent changes induced by VIP and PACAP on ADNP expression were developmentally dependent, and while stimulating expression in young astrocytes, an inhibition was demonstrated in older cultures. In conclusion, VIP, PACAP and the VPAC2 receptor may all contribute to the regulation of ADNP gene expression in the developing astrocyte.
KW - Activity-dependent neuroprotective protein
KW - Pituitary adenylate cyclase-activating polypeptide
KW - VIP/PACAP receptors VPAC1, VPAC2 and PAC1
KW - Vasoactive intestinal peptide
UR - http://www.scopus.com/inward/record.url?scp=7044260591&partnerID=8YFLogxK
U2 - 10.1016/j.regpep.2004.05.021
DO - 10.1016/j.regpep.2004.05.021
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:7044260591
SN - 0167-0115
VL - 123
SP - 33
EP - 41
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 1-3 SPEC. ISS.
ER -