Differential molecular regulation of processing and membrane expression of Type-I BMP receptors: implications for signaling

Tal Hirschhorn, Michal Levi-Hofman, Oded Danziger, Nechama I. Smorodinsky, Marcelo Ehrlich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The Type-I bone morphogenetic protein receptors (BMPRs), BMPR1A and BMPR1B, present the highest sequence homology among BMPRs, suggestive of functional similitude. However, sequence elements within their extracellular domain, such as signal sequence or N-glycosylation motifs, may result in differential regulation of biosynthetic processing and trafficking and in alterations to receptor function. We show that (i) BMPR1A and the ubiquitous isoform of BMPR1B differed in mode of translocation into the endoplasmic reticulum; and (ii) BMPR1A was N-glycosylated while BMPR1B was not, resulting in greater efficiency of processing and plasma membrane expression of BMPR1A. We further demonstrated the importance of BMPR1A expression and glycosylation in ES-2 ovarian cancer cells, where (i) CRISPR/Cas9-mediated knockout of BMPR1A abrogated BMP2-induced Smad1/5/8 phosphorylation and reduced proliferation of ES-2 cells and (ii) inhibition of N-glycosylation by site-directed mutagenesis, or by tunicamycin or 2-deoxy-d-glucose treatments, reduced biosynthetic processing and plasma membrane expression of BMPR1A and BMP2-induced Smad1/5/8 phosphorylation.

Original languageEnglish
Pages (from-to)2645-2662
Number of pages18
JournalCellular and Molecular Life Sciences
Volume74
Issue number14
DOIs
StatePublished - 1 Jul 2017

Keywords

  • Bone morphogenetic protein
  • Disulfide bond formation
  • ER translocation
  • Intracellular localization
  • N-Glycosylation
  • Ovarian cancer

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