Differential inhibition of the release of endogenous and newly synthesized acetylcholine from Torpedo synaptosomes by presynaptic muscarinic receptors

S. Luz, I. Pinchasi, D. M. Michaelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of Torpedo presynaptic muscarinic acetylcholine (ACh) receptors with the agonist oxotremorine (20 μM) results in the inhibition of Ca2+-dependent release of endogenous ACh from Torpedo synaptosomes. This effect is reversed by the muscarinic antagonist atropine (1 μM) which, by itself, has no effect. In contrast, under the same conditions the amount of newly synthesized radiolabeled [3H]ACh released is not affected by muscarinic ligands. These findings suggest that presynaptic muscarinic inhibition in the Torpedo is due to interference with the mobilization of ACh from a storage pool.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalFEBS Letters
Volume164
Issue number1
DOIs
StatePublished - 28 Nov 1983

Funding

FundersFunder number
US-Israel Binational ScienceF oundation
Muscular Dystrophy Association
United States-Israel Binational Science Foundation2410

    Keywords

    • Acetylcholine
    • Muscarinic acetylcholine receptor
    • Presynaptic inhibition
    • Synaptosome
    • Torpedo

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