TY - JOUR
T1 - Differential expression of renal growth hormone receptor and its binding protein in experimental diabetes mellitus
AU - Landau, D.
AU - Domene, H.
AU - Flyvbjerg, A.
AU - Grenbaek, H.
AU - Roberts, Jr
AU - Argov, S.
AU - LeRoith, D.
N1 - Funding Information:
We thank Dr W.R. Baumbach, from the American Cyanamide Co., Princeton, NJ, USA, for the supply of the GHBP monoclonal antibody, and Dr L. Mathews (San Diego, CA, USA), for providing the rat GHR cDNA clone. We are grateful to Kirsten Nyborg and Karen Mathiesen for excellent technical assistance. D.L. is a recipient of a grant-in-aid of the Chief Scientist of the Israeli Ministry of Health. This study was also supported by grants from the Danish Diabetes Association, the Danish Medical Research Council, the Ruth Konig Petersen Foundation, the Novo Foundation, the Nordic Insulin Foundation, the Aage Louis-Hansen Memorial Foundation and Institute of Experimental Clinical Research, University of Aarhus, Denmark.
PY - 1998
Y1 - 1998
N2 - Growth hormone (GH) may have a role in the development of diabetic nephropathy. The effect of experimental diabetes on renal expression of the growth hormone receptor gene products, including the receptor itself (GHR) and its binding protein (GHBP) was examined. Adult female rats received i.v. streptozotocin and were killed at 7, 30, 90 and 180 days after the induction of diabetes. Diabetic animals had a pronounced increase in kidney weight and progressive albuminuria. In renal cortex, no change was seen in GHR mRNA levels throughout the observation period of 6 months, while a significant increase in cortical GHBP mRNA levels was observed after 1 month of diabetes and sustained for the rest of the study period. Immunohistochemical analysis of kidney sections revealed a stronger staining for GHBP at the cortical and inner medullary areas in the diabetic animals. These data indicate that although the GHR and GHBP mRNAs originate from the same gene, their renal levels are differentially regulated during the development of experimental diabetic kidney disease, suggesting a functional role for GHBP.
AB - Growth hormone (GH) may have a role in the development of diabetic nephropathy. The effect of experimental diabetes on renal expression of the growth hormone receptor gene products, including the receptor itself (GHR) and its binding protein (GHBP) was examined. Adult female rats received i.v. streptozotocin and were killed at 7, 30, 90 and 180 days after the induction of diabetes. Diabetic animals had a pronounced increase in kidney weight and progressive albuminuria. In renal cortex, no change was seen in GHR mRNA levels throughout the observation period of 6 months, while a significant increase in cortical GHBP mRNA levels was observed after 1 month of diabetes and sustained for the rest of the study period. Immunohistochemical analysis of kidney sections revealed a stronger staining for GHBP at the cortical and inner medullary areas in the diabetic animals. These data indicate that although the GHR and GHBP mRNAs originate from the same gene, their renal levels are differentially regulated during the development of experimental diabetic kidney disease, suggesting a functional role for GHBP.
UR - http://www.scopus.com/inward/record.url?scp=0032454880&partnerID=8YFLogxK
U2 - 10.1016/S1096-6374(98)80320-1
DO - 10.1016/S1096-6374(98)80320-1
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C2 - 10990443
AN - SCOPUS:0032454880
SN - 1096-6374
VL - 8
SP - 39
EP - 45
JO - Growth Hormone and IGF Research
JF - Growth Hormone and IGF Research
IS - 1
ER -
