Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells

Lina Wasserman; Jardena Nordenberg; Einat Beery; Alexander A. Deutsch; Abraham Novogrodsky

doi:10.1159/000163418

Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells

Lina Wasserman^*, Jardena Nordenberg, Einat Beery, Alexander A. Deutsch, Abraham Novogrodsky

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semisolid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and y-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in y-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.

Original language	English
Pages (from-to)	189-193
Number of pages	5
Journal	Pathobiology
Volume	55
Issue number	4
DOIs	https://doi.org/10.1159/000163418
State	Published - 1987
Externally published	Yes

Keywords

Alkaline phosphatase
Differentiation
MCF-7 breast cancer cells
γ-Glutamyl transpeptidase

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title = "Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells",

abstract = "Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semisolid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and y-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in y-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.",

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year = "1987",

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T1 - Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells

AU - Wasserman, Lina

AU - Nordenberg, Jardena

AU - Beery, Einat

AU - Deutsch, Alexander A.

AU - Novogrodsky, Abraham

PY - 1987

Y1 - 1987

N2 - Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semisolid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and y-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in y-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.

AB - Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semisolid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and y-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in y-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.

KW - Alkaline phosphatase

KW - Differentiation

KW - MCF-7 breast cancer cells

KW - γ-Glutamyl transpeptidase

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Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells

Abstract

Keywords

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