Differential effects of 1,25-(OH)2D3 on acyl hydrolase and cycloxygenase activities in interleukin 1β-stimulated human synovial fibroblast cultures

F. A. Meyer, I. Yaron, N. Kamin-Belsky, M. Yaron, A. Raz

Research output: Contribution to journalArticlepeer-review

Abstract

Effects of proinflammatory cytokines on synovial fibroblasts are considered to play a role in the accumulation of prostaglandin E2 (PGE2) in the inflamed joint in rheumatoid arthritis. We have previously shown that interleukin 1β (IL-1β) induces PGE2 production in synovial fibroblast cultures and that this effect is suppressed by the active metabolite of vitamin D3, 1,25-(OH)2D3. To study the mechanism of the inhibitory action of 1,25-(OH)2D3, its effect on acyl hydrolase (arachidonic acid (AA) release) and cyclooxygenase (COX) activities were investigated. Our findings indicate that IL-1β caused an increase in AA release and COX activity and that in the presence of 1,25(OH)2D3 AA release, but not COX activity, is suppressed. In comparison, dexamethasone, which also inhibits IL-1β induction of PGE2, had inhibitory effects on both parameters.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume55
Issue number3
DOIs
StatePublished - Sep 1996

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