Differential Effect of Actomyosin Relaxation on the Dynamic Properties of Focal Adhesion Proteins

Irena Lavelin, Haguy Wolfenson, Israel Patla, Yoav I. Henis, Ohad Medalia, Tova Volberg, Ariel Livne, Zvi Kam, Benjamin Geiger

Research output: Contribution to journalArticlepeer-review

Abstract

Treatment of cultured cells with inhibitors of actomyosin contractility induces rapid deterioration of stress fibers, and disassembly of the associated focal adhesions (FAs). In this study, we show that treatment with the Rho kinase inhibitor Y-27632, which blocks actomyosin contractility, induces disarray in the FA-associated actin bundles, followed by the differential dissociation of eight FA components from the adhesion sites. Live-cell microscopy indicated that the drug triggers rapid dissociation of VASP and zyxin from FAs (τ values of 7-8 min), followed by talin, paxillin and ILK (τ ~16 min), and then by FAK, vinculin and kindlin-2 (τ = 25-28 min). Examination of the molecular kinetics of the various FA constituents, using Fluorescence Recovery After Photobleaching (FRAP), in the absence of or following short-term treatment with the drug, revealed major changes in the kon and koff values of the different proteins tested, which are in close agreement with their differential dissociation rates from the adhesion sites. These findings indicate that mechanical, actomyosin-generated forces differentially regulate the molecular kinetics of individual FA-associated molecules, and thereby modulate FA composition and stability.

Original languageEnglish
Article numbere73549
JournalPLoS ONE
Volume8
Issue number9
DOIs
StatePublished - 9 Sep 2013

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