Differential Cell Death and Regrowth of Dermal Fibroblasts and Keratinocytes After Application of Pulsed Electric Fields

Bodhisatwa Das, Anil Shrirao, Alexander Golberg, Francois Berthiaume, Rene Schloss, Martin L. Yarmush

Research output: Contribution to journalArticlepeer-review

Abstract

Background: High-powered pulsed electric fields (PEF) may be used for tissue debridement and disinfection, while lower PEF intensities may stimulate beneficial cellular responses for wound healing. We investigated the dual effects of nonuniform PEF on cellular death and stimulation. Methods: Dermal fibroblast or keratinocyte monolayers were exposed to PEF induced by two needle electrodes (2 mm apart). Voltages (100-600 V; 1 Hz; 70 micros pulse width; 90 pulses/cycle) were applied between the two electrodes. Controls consisted of similar monolayers subjected to a scratch mechanical injury. Results: Cell growth and closure of the cell-free gap was faster in PEF-treated cell monolayers versus scratched ones. Media conditioned from cells pre-exposed to PEF, when applied to responder cells, stimulated greater proliferation than media from scratched monolayers. Conclusions: PEF treatment causes the release of soluble factors that promote cell growth, and thus may play a role in the accelerated healing of wounds post PEF.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalBioelectricity
Volume2
Issue number2
DOIs
StatePublished - Jun 2020

Keywords

  • pulsed electric fields
  • skin
  • wound healing

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