Different routes of bone morphogenic protein (BMP) receptor endocytosis influence BMP signaling

Anke Hartung, Keren Bitton-Worms, Maya Mouler Rechtman, Valeska Wenzel, Jan H. Boergermann, Sylke Hassel, Yoav I. Henis, Petra Knaus*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Endocytosis is important for a variety of functions in eukaryotic cells, including the regulation of signaling cascades via transmembrane receptors. The internalization of bone morphogenetic protein (BMP) receptor type I (BRI) and type II (BRII) and its relation to signaling were largely unexplored. Here, we demonstrate that both receptor types undergo constitutive endocytosis via clathrin-coated pits (CCPs) but that only BRII undergoes also caveola-like internalization. Using several complementary approaches, we could show that (i) BMP-2-mediated Smad1/5 phosphorylation occurs at the plasma membrane in nonraft regions, (ii) continuation of Smad signaling resulting in a transcriptional response requires endocytosis via the clathrin-mediated route, and (iii) BMP signaling leading to alkaline phosphatase induction initiates from receptors that fractionate into cholesterol-enriched, detergent-resistant membranes. Furthermore, we show that BRII interacts with Eps15R, a constitutive component of CCPs, and with caveolin-1, the marker protein of caveolae. Taken together, the localization of BMP receptors in distinct membrane domains is prerequisite to their taking different endocytosis routes with specific impacts on Smad-dependent and Smad-independent signaling cascades.

Original languageEnglish
Pages (from-to)7791-7805
Number of pages15
JournalMolecular and Cellular Biology
Volume26
Issue number20
DOIs
StatePublished - Oct 2006

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