Differences in metabolite content between intact pancreases and their perchloric acid extracts. A 2D ¹H/³¹P correlation NMR study

Perchloric and hydrochloric acid extracts of intact pancreases from healthy rats and from rats with experimental acute pancreatitis were analysed using 2D ¹H/³¹P correlation NMR spectroscopy. Major differences were found in the 2D maps between the extracts and the intact tissues. In the case of the intact diseased pancreas the prominent ³¹P signal at the phosphodiester region has been assigned in our previous work as lecithin/taurocholate complex. However, the signal found in the same chemical shift region in extracts of diseased pancreases as well as healthy ones, is identified here as the phosphodiester residue of oligoribonucleotides. In these extracts additional ³¹P signals were found and assigned as phosphomonoester and phosphodiester hydrolysis products of RNA. The amounts of these compounds, as a function of the acid concentrations were determined, and conditions for their minimization were defined.