TY - JOUR
T1 - Dielectric screening of early differentiation patterns in mesenchymal stem cells induced by steroid hormones
AU - Ron, Amit
AU - Shur, Irena
AU - Daniel, Ramiz
AU - Singh, Ragini Raj
AU - Fishelson, Nick
AU - Croitoru, Nathan
AU - Benayahu, Dafna
AU - Shacham-Diamand, Yosi
PY - 2010/6
Y1 - 2010/6
N2 - In the framework of this study, target identification and localization of differentiation patterns by means of dielectric spectroscopy is presented. Here, a primary pre-osteoblastic bone marrow-derived MBA-15 cellular system was used to study the variations in the dielectric properties of mesenchymal stem cells while exposed to differentiation regulators. Using the fundamentals of mixed dielectric theories combined with finite numerical tools, the permittivity spectra of MBA-15 cell suspensions have been uniquely analyzed after being activated by steroid hormones to express osteogenic phenotypes. Following the spectral analysis, significant variations were revealed in the dielectric properties of the activated cells in comparison to the untreated populations. Based on the differentiation patterns of MBA-15, the electrical modifications were found to be highly correlated with the activation of specific cellular mechanisms which directly react to the hormonal inductions. In addition, by describing the dielectric dispersion in terms of transfer functions, it is shown that the spectral perturbations are well adapted to variations in the electrical characteristics of the cells. The reported findings vastly emphasize the tight correlation between the cellular and electrical state of the differentiated cells. It therefore emphasizes the vast abilities of impedance-based techniques as potential screening tools for stem cell analysis.
AB - In the framework of this study, target identification and localization of differentiation patterns by means of dielectric spectroscopy is presented. Here, a primary pre-osteoblastic bone marrow-derived MBA-15 cellular system was used to study the variations in the dielectric properties of mesenchymal stem cells while exposed to differentiation regulators. Using the fundamentals of mixed dielectric theories combined with finite numerical tools, the permittivity spectra of MBA-15 cell suspensions have been uniquely analyzed after being activated by steroid hormones to express osteogenic phenotypes. Following the spectral analysis, significant variations were revealed in the dielectric properties of the activated cells in comparison to the untreated populations. Based on the differentiation patterns of MBA-15, the electrical modifications were found to be highly correlated with the activation of specific cellular mechanisms which directly react to the hormonal inductions. In addition, by describing the dielectric dispersion in terms of transfer functions, it is shown that the spectral perturbations are well adapted to variations in the electrical characteristics of the cells. The reported findings vastly emphasize the tight correlation between the cellular and electrical state of the differentiated cells. It therefore emphasizes the vast abilities of impedance-based techniques as potential screening tools for stem cell analysis.
KW - Analog electric circuit
KW - Dielectric spectroscopy
KW - Finite numerical analysis
KW - Mesenchymal stem cells
KW - Steroid hormones
UR - http://www.scopus.com/inward/record.url?scp=77949567311&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2009.09.008
DO - 10.1016/j.bioelechem.2009.09.008
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AN - SCOPUS:77949567311
SN - 1567-5394
VL - 78
SP - 161
EP - 172
JO - Bioelectrochemistry
JF - Bioelectrochemistry
IS - 2
ER -