Dielectric dispersion of suspended cells using 3D reconstructed morphology model

Amit Ron, Ragini Raj Singh, Nick Fishelson, Irena Shur, Rina Socher, Nathan Croitoriu, Dafna Benayahu, Yosi Shacham-Diamand

Research output: Contribution to journalArticlepeer-review

Abstract

In the framework of this study, novel method for dispersion analysis of cellular suspensions is presented. The method is fundamentally based on the ability to reconstruct the exact 3D morphology of a given cell with resolution accuracy of few nanometers using AFM imaging. By applying a reverse engineering approach, the morphology of the cell is constructed based on a set of measured spatial points that describes its geometry. The permittivity spectrum of the reconstructed cell is then directly derived based on computational solution of complex potential problem using 3D Boundary Element Method. The applicability of the method is demonstrated both theoretically and experimentally with tight comparison to the well known shell models. This comparison reveals significant deviations between the models, and hence, emphasises the vast effect of morphology in dispersion analysis of cellular suspensions.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalBioelectrochemistry
Volume75
Issue number2
DOIs
StatePublished - Jun 2009

Keywords

  • Atomic force microscopy
  • Complex permittivity
  • Dielectric dispersion
  • Finite numerical analysis

Fingerprint

Dive into the research topics of 'Dielectric dispersion of suspended cells using 3D reconstructed morphology model'. Together they form a unique fingerprint.

Cite this