Dibutyl phthalate impairs steroidogenesis and a subset of LH-dependent genes in cultured human mural granulosa cell in vitro

Michal Adir, Catherine M.H. Combelles, Abdallah Mansur, Libby Ophir, Ariel Hourvitz, Raoul Orvieto, Jehoshua Dor, Ronit Machtinger

Research output: Contribution to journalArticlepeer-review

Abstract

Exposure to di-butyl phthalate (DBP) exerts negative effects on female fertility in animal models, but human studies remain limited. Here, the effects of DBP exposure on mural granulosa cell function were investigated in primary cultures from women undergoing in vitro fertilization. Cultured cells treated with various doses of DBP (0, 0.01 μg/mL, 0.1 μg/mL, 1 μg/mL, 10 μg/mL, or 100 μg/mL) for 48 h were assessed using enzyme-linked immunosorbent assay and qRT-PCR. Treatment with 100 μg/mL DBP resulted in significantly lower 17β-estradiol and progesterone production (p < 0.01). It also resulted in altered mRNA expression of steroidogenic, angiogenic, and epidermal growth factor-like growth factor genes: CYP11A1 (p < 0.001), CYP19A1 (aromatase) (p < 0.001), VEGF-A (p < 0.02), BTC (p = 0.009), and EREG (p = 0.04). StAR expression was impaired after exposure to both 10 and 100 μg/mL (p < 0.03 and p < 0.001, respectively). Our results indicate that in vitro exposure of granulosa cells to high doses of DBP alters cell functions.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalReproductive Toxicology
Volume69
DOIs
StatePublished - 1 Apr 2017

Keywords

  • Dibutyl phthalate
  • EGF–like factors
  • Endocrine disruptors
  • Granulosa cells
  • Steroidogenesis

Fingerprint

Dive into the research topics of 'Dibutyl phthalate impairs steroidogenesis and a subset of LH-dependent genes in cultured human mural granulosa cell in vitro'. Together they form a unique fingerprint.

Cite this