TY - JOUR
T1 - Diamide, a new reagent for the intracellular oxidation of glutathione to the disulfide
AU - Kosower, Nechama S.
AU - Kosower, Edward M.
AU - Wertheim, Bilha
AU - Correa, Walter S.
N1 - Funding Information:
Career Development Awardee of the National Institutes of Health. Permanent address: Dept. of Medioine, Albert Einstein College of Mediclne, Bronx, N. Y. 10461 Dept. of Biologloal Ultrartruoture. National Solenoe Foundation Laior Pootdoatoral Fellow 1963-9. Permanent address: Dept. of Chemistry, State University of New York, Stony Brook, N, Y. 11799, Dept. of Biophysios Bookefeller Fouudatioa Predootoral Fellow 1967-1970 Support from the National Institutes of Health, The Army Rerearoh Offioe (Durham) and the Petroleum Hesearoh Fund ie gratefully aoknowledged.
PY - 1969/11/6
Y1 - 1969/11/6
N2 - A new thiol-oxidizing agent, diamide, (CH3)2NCONNCON(CH3)2, is shown to stoichiometrically oxidize glutathione (GSH) within the human red blood cell to the disulfide (GSSG). Conversion of GSH to GSSG is very rapid, even at 1-40. No inter ference with cellular function is observed as shown by almost complete regeneration of GSH after incubation with glucose at 370, and no alteration in hemoglobin, osmotic fragility or cell density is found.
AB - A new thiol-oxidizing agent, diamide, (CH3)2NCONNCON(CH3)2, is shown to stoichiometrically oxidize glutathione (GSH) within the human red blood cell to the disulfide (GSSG). Conversion of GSH to GSSG is very rapid, even at 1-40. No inter ference with cellular function is observed as shown by almost complete regeneration of GSH after incubation with glucose at 370, and no alteration in hemoglobin, osmotic fragility or cell density is found.
UR - http://www.scopus.com/inward/record.url?scp=0014666236&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(69)90850-X
DO - 10.1016/0006-291X(69)90850-X
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AN - SCOPUS:0014666236
SN - 0006-291X
VL - 37
SP - 593
EP - 596
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -