Diamide, a new reagent for the intracellular oxidation of glutathione to the disulfide

Nechama S. Kosower*, Edward M. Kosower, Bilha Wertheim, Walter S. Correa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

431 Scopus citations

Abstract

A new thiol-oxidizing agent, diamide, (CH3)2NCONNCON(CH3)2, is shown to stoichiometrically oxidize glutathione (GSH) within the human red blood cell to the disulfide (GSSG). Conversion of GSH to GSSG is very rapid, even at 1-40. No inter ference with cellular function is observed as shown by almost complete regeneration of GSH after incubation with glucose at 370, and no alteration in hemoglobin, osmotic fragility or cell density is found.

Original languageEnglish
Pages (from-to)593-596
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume37
Issue number4
DOIs
StatePublished - 6 Nov 1969
Externally publishedYes

Funding

FundersFunder number
Army Rerearoh Offioe
Petroleum Hesearoh Fund
National Institutes of Health

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