TY - JOUR
T1 - Diagnosis and classification of arnyloidosis by an immuno-histological method
AU - Levo, Y.
AU - Livni, N.
AU - Laufer, A.
PY - 1982
Y1 - 1982
N2 - Following the recent classification of amylodosis by the amyloid proteins involved, we decided to approach its tissue diagnosis and chemical classification by an immunohistological method. Post-mortem tissue specimens from various organs of patients with primary and secondary amyloidosis were examined by the immunoperoxidase technique with specific antibodies against proteins AL and AA which respectively characterize primary and secondary amyloidosis. The major advantage of this technique is that it can be applied to formalin fixed, paraffin embedded tissues. The immunoperoxidase technique proved to be extremely sensitive for the detection of amyloid deposits. Moreover, the deposited proteins could be clearly characterized as AL or AA by the specific anti-sera. We therefore believe that the immunooperoxidase technique is extremely useful for the early tissue diagnosis and chemical classification of amyloidosis.
AB - Following the recent classification of amylodosis by the amyloid proteins involved, we decided to approach its tissue diagnosis and chemical classification by an immunohistological method. Post-mortem tissue specimens from various organs of patients with primary and secondary amyloidosis were examined by the immunoperoxidase technique with specific antibodies against proteins AL and AA which respectively characterize primary and secondary amyloidosis. The major advantage of this technique is that it can be applied to formalin fixed, paraffin embedded tissues. The immunoperoxidase technique proved to be extremely sensitive for the detection of amyloid deposits. Moreover, the deposited proteins could be clearly characterized as AL or AA by the specific anti-sera. We therefore believe that the immunooperoxidase technique is extremely useful for the early tissue diagnosis and chemical classification of amyloidosis.
KW - Amyloidosis
KW - Immunodiagnosis
KW - Immunoperoxidase technique
UR - http://www.scopus.com/inward/record.url?scp=0020312467&partnerID=8YFLogxK
U2 - 10.1016/S0344-0338(82)80050-2
DO - 10.1016/S0344-0338(82)80050-2
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AN - SCOPUS:0020312467
VL - 175
SP - 373
EP - 379
JO - Pathology Research and Practice
JF - Pathology Research and Practice
SN - 0344-0338
IS - 4
ER -