TY - JOUR
T1 - Developmental regulation of the rat insulin-like growth factor I receptor gene
AU - Werner, H.
AU - Woloschak, M.
AU - Adamo, M.
AU - Shen-Orr, Z.
AU - Roberts, C. T.
AU - LeRoith, D.
PY - 1989
Y1 - 1989
N2 - We have invetigated the developmental regulation of the rat insulin-like growth factor I (IGF-I) receptor gene in various tissue using a sensitive and specific solution hybridization/RNase protection assay. For this purpose we characterized rat IGF-I receptor cDNAs that were cloned from a simian virus 40-transformed rat granulosa cell cDNA library. The specific cDNA clone used in these studies encoded the putative signal peptide and the first 53 amino acids of the α subunit and was ~94% homologous to its human counterpart. IGF-I receptor gene expression was studied during the perinatal period and at various intervals until early adulthood. Overall, steady-state IGF-I receptor mRNA levels decreased dramatically during postnatal development; however, the extent of the decrease differed among the various tissues studied. In contrast to receptor mRNA levels, IGF-I mRNA levels increased in some of the same tissues. The molecular mechanisms underlying this apparent divergent transcriptional control of the IGF-I and IGF-I receptor genes warrant further study.
AB - We have invetigated the developmental regulation of the rat insulin-like growth factor I (IGF-I) receptor gene in various tissue using a sensitive and specific solution hybridization/RNase protection assay. For this purpose we characterized rat IGF-I receptor cDNAs that were cloned from a simian virus 40-transformed rat granulosa cell cDNA library. The specific cDNA clone used in these studies encoded the putative signal peptide and the first 53 amino acids of the α subunit and was ~94% homologous to its human counterpart. IGF-I receptor gene expression was studied during the perinatal period and at various intervals until early adulthood. Overall, steady-state IGF-I receptor mRNA levels decreased dramatically during postnatal development; however, the extent of the decrease differed among the various tissues studied. In contrast to receptor mRNA levels, IGF-I mRNA levels increased in some of the same tissues. The molecular mechanisms underlying this apparent divergent transcriptional control of the IGF-I and IGF-I receptor genes warrant further study.
UR - http://www.scopus.com/inward/record.url?scp=0001758651&partnerID=8YFLogxK
U2 - 10.1073/pnas.86.19.7451
DO - 10.1073/pnas.86.19.7451
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0001758651
SN - 0027-8424
VL - 86
SP - 7451
EP - 7455
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 19
ER -