Developmental expression of voltage-sensitive K+ channels in mouse skeletal muscle and C2C12 cells

Florian Lesage; Bernard Attali; Michel Lazdunski; Jacques Barhanin

doi:10.1016/0014-5793(92)81320-L

Developmental expression of voltage-sensitive K⁺ channels in mouse skeletal muscle and C₂C₁₂ cells

Florian Lesage^*, Bernard Attali, Michel Lazdunski, Jacques Barhanin

^*Corresponding author for this work

Inst. Pharmacologie Molec. et Cell.

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The developmental expression of voltage-sensitive K⁺ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C₂C₁₂ has been used to study expression of voltage-sensitive K⁺ channels during in vitro myotube differentiation. Different voltage-sensitive K⁺ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K⁺ channel or IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.

Original language	English
Pages (from-to)	162-166
Number of pages	5
Journal	FEBS Letters
Volume	310
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)81320-L
State	Published - 28 Sep 1992
Externally published	Yes

Keywords

Development
Differentiation
Ionic channel
Myoblast
Myotube

Access to Document

10.1016/0014-5793(92)81320-L

Cite this

@article{ce68b5a2779643c2a495a9754def51ae,

title = "Developmental expression of voltage-sensitive K+ channels in mouse skeletal muscle and C2C12 cells",

abstract = "The developmental expression of voltage-sensitive K+ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C2C12 has been used to study expression of voltage-sensitive K+ channels during in vitro myotube differentiation. Different voltage-sensitive K+ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K+ channel or IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.",

keywords = "Development, Differentiation, Ionic channel, Myoblast, Myotube",

author = "Florian Lesage and Bernard Attali and Michel Lazdunski and Jacques Barhanin",

year = "1992",

month = sep,

day = "28",

doi = "10.1016/0014-5793(92)81320-L",

language = "אנגלית",

volume = "310",

pages = "162--166",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Developmental expression of voltage-sensitive K+ channels in mouse skeletal muscle and C2C12 cells

AU - Lesage, Florian

AU - Attali, Bernard

AU - Lazdunski, Michel

AU - Barhanin, Jacques

PY - 1992/9/28

Y1 - 1992/9/28

N2 - The developmental expression of voltage-sensitive K+ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C2C12 has been used to study expression of voltage-sensitive K+ channels during in vitro myotube differentiation. Different voltage-sensitive K+ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K+ channel or IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.

AB - The developmental expression of voltage-sensitive K+ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C2C12 has been used to study expression of voltage-sensitive K+ channels during in vitro myotube differentiation. Different voltage-sensitive K+ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K+ channel or IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.

KW - Development

KW - Differentiation

KW - Ionic channel

KW - Myoblast

KW - Myotube

UR - http://www.scopus.com/inward/record.url?scp=0026640594&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(92)81320-L

DO - 10.1016/0014-5793(92)81320-L

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0026640594

SN - 0014-5793

VL - 310

SP - 162

EP - 166

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Developmental expression of voltage-sensitive K⁺ channels in mouse skeletal muscle and C₂C₁₂ cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this