TY - JOUR
T1 - Developmental expression of the α receptor for platelet-derived growth factor, which is deleted in the embryonic lethal Patch mutation
AU - Orr-Urtreger, Avi
AU - Bedford, Mark T.
AU - Do, Myoung Sool
AU - Eisenbach, Lea
AU - Lonai, Peter
PY - 1992/5
Y1 - 1992/5
N2 - The α receptor of PDGF (Pdgfra) is expressed in primitive endoderm and mesoderm derivatives throughout embryogenesis. In the early primitive streak stage the gene is transcribed in the visceral and parietal endoderm. Later it is expressed in the presomitic mesoderm, yolk sac and amnion. During somitogenesis its transcription localizes to the heart and the somites. Subsequently, it is transcribed in the dermatome, the sclerotome, the developing limb and in various mesenchymal tissues of visceral organs. Its wild-type expression pattern correlates well with the phenotype of homozygous mutant Patch (Ph) embryos, where the Pdgfra gene is deleted. The Ph phenotype is first detectable at the primitive streak stage with convoluted and hypertrophic visceral yolk sac, deformed neural plate and disorganized or missing mesoderm. Most Ph/Ph embryos die before the 11th day of gestation. Those that survive till early organogenesis are very small, have hypertrophic yolk sacs, small and undifferentiated somites, convoluted neural tubes, large heart and pericardium, rudimentary limb buds and branchial arches. Our observations together suggest that the α PDGF receptor may be required for the normal development of visceral endoderm and mesoderm derivatives.
AB - The α receptor of PDGF (Pdgfra) is expressed in primitive endoderm and mesoderm derivatives throughout embryogenesis. In the early primitive streak stage the gene is transcribed in the visceral and parietal endoderm. Later it is expressed in the presomitic mesoderm, yolk sac and amnion. During somitogenesis its transcription localizes to the heart and the somites. Subsequently, it is transcribed in the dermatome, the sclerotome, the developing limb and in various mesenchymal tissues of visceral organs. Its wild-type expression pattern correlates well with the phenotype of homozygous mutant Patch (Ph) embryos, where the Pdgfra gene is deleted. The Ph phenotype is first detectable at the primitive streak stage with convoluted and hypertrophic visceral yolk sac, deformed neural plate and disorganized or missing mesoderm. Most Ph/Ph embryos die before the 11th day of gestation. Those that survive till early organogenesis are very small, have hypertrophic yolk sacs, small and undifferentiated somites, convoluted neural tubes, large heart and pericardium, rudimentary limb buds and branchial arches. Our observations together suggest that the α PDGF receptor may be required for the normal development of visceral endoderm and mesoderm derivatives.
KW - Embryogenesis
KW - Embryonic lethality
KW - In situ hybridization
KW - PDGF receptor
KW - Ph mutation
UR - http://www.scopus.com/inward/record.url?scp=0026695298&partnerID=8YFLogxK
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C2 - 1322271
AN - SCOPUS:0026695298
SN - 0950-1991
VL - 115
SP - 289
EP - 303
JO - Development (Cambridge)
JF - Development (Cambridge)
IS - 1
ER -