Development of Postmeiotic Cells in Vitro from Spermatogonial Cells of Prepubertal Cancer Patients

Maram Abofoul-Azab, Ali Abumadighem, Eitan Lunenfeld, Joseph Kapelushnik, Qinghua Shi, Haim Pinkas, Mahmoud Huleihel

Research output: Contribution to journalArticlepeer-review

Abstract

Aggressive chemotherapy in childhood often results in testicular damage and consequently jeopardizes future fertility. The presence of spermatogonial cells (SPGCs) in the testes of prepubertal cancer patient boys (PCPBs) can be used to develop future strategies for male fertility preservation. In the present study, we examined the presence of SPGCs in testes of chemotherapy-treated PCPBs and their ability to develop spermatogenesis in vitro using a three-dimensional culture system. Seven testicular biopsies were obtained from chemotherapy-treated PCPBs and one from a patient with β-thalassemia major. Isolated testicular cells were cultured in a methylcellulose culture system (MCS)-containing StemPro enriched with growth factors for 5-15 weeks. The presence of premeiotic, meiotic, and postmeiotic cells was examined by immunofluorescence staining and/or reverse transcription-polymerase chain reaction (RT-PCR) analysis. We observed SPGCs in the examined testicular biopsies. Isolated testicular cells cultured in MCS developed into colonies and contained premeiotic, meiotic, and postmeiotic cells. Furthermore, we identified sperm-like cells that had developed from testicular cells of a PCPB. Our results demonstrate, for the first time, the presence of biologically active SPGCs in testicular biopsies of chemotherapy-treated PCPBs and their capacity to develop in vitro to different stages of spermatogenesis, including the generation of sperm-like cells. This study may open the way for new therapeutic strategies for fertility preservation of PCPBs and for azoospermic patients.

Original languageEnglish
Pages (from-to)1007-1020
Number of pages14
JournalStem Cells and Development
Volume27
Issue number15
DOIs
StatePublished - 1 Aug 2018
Externally publishedYes

Keywords

  • chemotherapy
  • in vitro culture
  • male infertility
  • prepubertal cancer patient boys
  • spermatogenesis
  • spermatogonial cells

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