TY - JOUR
T1 - Development of a quantitative optical biochip based on a double integrating sphere system that determines absolute photon number in bioluminescent solution
T2 - Application to quantum yield scale realization
AU - Daniel, Ramiz
AU - Almog, Ronen
AU - Sverdlov, Yelena
AU - Yagurkroll, Sharon
AU - Belkin, Shimshon
AU - Shacham-Diamand, Yosi
PY - 2009/6/10
Y1 - 2009/6/10
N2 - We report a new design of an optical biochip based on a double integrating sphere system to quantify the absolute number of the emitted photons or the total photon flux by a whole cell bioluminescent biosensor, for water toxicity detection, based on genetically engineered Escherichia coli bacteria carrying a recA::luxCDABE promoter-reporter fusion. The new design of the double integrating sphere system does not require any external standard light source for calibration of the tested bioluminescent solution and allows a direct determination of the total photon flux of the bioluminescent solution. In our design, we required that the two spheres are symmetric (have the same radius and reflectance) with a surface area larger than the cut cap area between the spheres.
AB - We report a new design of an optical biochip based on a double integrating sphere system to quantify the absolute number of the emitted photons or the total photon flux by a whole cell bioluminescent biosensor, for water toxicity detection, based on genetically engineered Escherichia coli bacteria carrying a recA::luxCDABE promoter-reporter fusion. The new design of the double integrating sphere system does not require any external standard light source for calibration of the tested bioluminescent solution and allows a direct determination of the total photon flux of the bioluminescent solution. In our design, we required that the two spheres are symmetric (have the same radius and reflectance) with a surface area larger than the cut cap area between the spheres.
UR - http://www.scopus.com/inward/record.url?scp=67650717989&partnerID=8YFLogxK
U2 - 10.1364/AO.48.003216
DO - 10.1364/AO.48.003216
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AN - SCOPUS:67650717989
SN - 1559-128X
VL - 48
SP - 3216
EP - 3224
JO - Applied Optics
JF - Applied Optics
IS - 17
ER -