TY - JOUR
T1 - Development and recovery of laser-induced retinal injury in rats
AU - Belokopytov, Mark
AU - Dubinsky, Galina
AU - Belkin, Michael
AU - Epstein, Yoram
AU - Rosner, Mordechai
PY - 2005
Y1 - 2005
N2 - Retinal photocoagulation lesions undergo primary and secondary degeneration followed by partial healing. This study follows the sequential changes in laser-induced retinal lesion over a time span of 60 days. Argon laser lesions (514 & 544 nm, 200 μm, 0.1 W, 0.05 sec) were created in 36 pigmented rats. Sections of the retinal lesions were evaluated by light microscopy 1, 24, 48, 72 hours, 20, and 60 days after the injury (six animals at each time point). The diameter of the lesion was equal to that of the laser spot Ih after irradiation and increased by 24h. It decreased later, slightly during the following 48h and significantly by 20 days. The destruction of photoreceptors was most severe after 24-48h. The nuclei in the outer-nuclear layer at the lesion site were pyknotic at one hour and disappeared later. Healing processes began 72h after the irradiation and was completed by 60 days. Filling-in by sliding of near nuclei was observed by the 60th day. Reversible changes were seen also in the retinal pigment epithelium (with formation of a plaque at 72h and its degradation later on) and in the choroid (disorganization of capillaries by 48h with later reorganization). In summary, the development of a laser-induced injury is gradual: photoreceptors are damaged first and than the damage spreads to other layers of retina and to areas adjacent the primary injury site. The expansion of the damage is stopped by 72h and the adjacent tissues start to fill the area of the lesion and remodel the retina.
AB - Retinal photocoagulation lesions undergo primary and secondary degeneration followed by partial healing. This study follows the sequential changes in laser-induced retinal lesion over a time span of 60 days. Argon laser lesions (514 & 544 nm, 200 μm, 0.1 W, 0.05 sec) were created in 36 pigmented rats. Sections of the retinal lesions were evaluated by light microscopy 1, 24, 48, 72 hours, 20, and 60 days after the injury (six animals at each time point). The diameter of the lesion was equal to that of the laser spot Ih after irradiation and increased by 24h. It decreased later, slightly during the following 48h and significantly by 20 days. The destruction of photoreceptors was most severe after 24-48h. The nuclei in the outer-nuclear layer at the lesion site were pyknotic at one hour and disappeared later. Healing processes began 72h after the irradiation and was completed by 60 days. Filling-in by sliding of near nuclei was observed by the 60th day. Reversible changes were seen also in the retinal pigment epithelium (with formation of a plaque at 72h and its degradation later on) and in the choroid (disorganization of capillaries by 48h with later reorganization). In summary, the development of a laser-induced injury is gradual: photoreceptors are damaged first and than the damage spreads to other layers of retina and to areas adjacent the primary injury site. The expansion of the damage is stopped by 72h and the adjacent tissues start to fill the area of the lesion and remodel the retina.
KW - Laser injury
KW - Retina
KW - Retinal healing
KW - Retinal injury
KW - Secondary degeneration
UR - http://www.scopus.com/inward/record.url?scp=21844438713&partnerID=8YFLogxK
U2 - 10.1117/12.583328
DO - 10.1117/12.583328
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AN - SCOPUS:21844438713
SN - 1605-7422
VL - 5688
SP - 312
EP - 319
JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
M1 - 56
T2 - Ophtalmic Technologies XV
Y2 - 22 January 2005 through 25 January 2005
ER -