Determination of pyruvate kinase activity by recording of pH changes

C. Carmeli; Y. Lifshitz

doi:10.1016/0003-2697(70)90455-0

Determination of pyruvate kinase activity by recording of pH changes

C. Carmeli^*, Y. Lifshitz

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Pyruvate kinase activity was determined by recording changes in pH and by recording titration of the hydroxyl ions released during the reaction. The same methods were also employed for determination of ADP and of phosphoenolpyruvate. The results obtained in the determination of pyruvate kinase activity by following the pH changes and by titration were compared to those obtained by the spectroscopic method using lactate dehydrogenase and NADH.

Original language	English
Pages (from-to)	309-312
Number of pages	4
Journal	Analytical Biochemistry
Volume	38
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(70)90455-0
State	Published - Dec 1970

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title = "Determination of pyruvate kinase activity by recording of pH changes",

abstract = "Pyruvate kinase activity was determined by recording changes in pH and by recording titration of the hydroxyl ions released during the reaction. The same methods were also employed for determination of ADP and of phosphoenolpyruvate. The results obtained in the determination of pyruvate kinase activity by following the pH changes and by titration were compared to those obtained by the spectroscopic method using lactate dehydrogenase and NADH.",

author = "C. Carmeli and Y. Lifshitz",

year = "1970",

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doi = "10.1016/0003-2697(70)90455-0",

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AB - Pyruvate kinase activity was determined by recording changes in pH and by recording titration of the hydroxyl ions released during the reaction. The same methods were also employed for determination of ADP and of phosphoenolpyruvate. The results obtained in the determination of pyruvate kinase activity by following the pH changes and by titration were compared to those obtained by the spectroscopic method using lactate dehydrogenase and NADH.

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Determination of pyruvate kinase activity by recording of pH changes

Abstract

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