Determination of hydroxypyrene trisulfonate by two wavelength excitation fluorescence using a one microliter capillary

Eli Flaxer; Boris Fisherman; Reuven Tirosh; Fernando Patolsky

doi:10.1080/10739149.2014.942916

Determination of hydroxypyrene trisulfonate by two wavelength excitation fluorescence using a one microliter capillary

Eli Flaxer^*, Boris Fisherman, Reuven Tirosh, Fernando Patolsky

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.

Original language	English
Pages (from-to)	627-634
Number of pages	8
Journal	Instrumentation Science and Technology
Volume	42
Issue number	6
DOIs	https://doi.org/10.1080/10739149.2014.942916
State	Published - 15 Nov 2014

Keywords

Capillaries
Fluorescence
Spectroscopy

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10.1080/10739149.2014.942916

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title = "Determination of hydroxypyrene trisulfonate by two wavelength excitation fluorescence using a one microliter capillary",

abstract = "Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.",

keywords = "Capillaries, Fluorescence, Spectroscopy",

author = "Eli Flaxer and Boris Fisherman and Reuven Tirosh and Fernando Patolsky",

note = "Publisher Copyright: Copyright {\textcopyright} Taylor & Francis Group, LLC.",

year = "2014",

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doi = "10.1080/10739149.2014.942916",

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AU - Flaxer, Eli

AU - Fisherman, Boris

AU - Tirosh, Reuven

AU - Patolsky, Fernando

PY - 2014/11/15

Y1 - 2014/11/15

N2 - Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.

AB - Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.

KW - Capillaries

KW - Fluorescence

KW - Spectroscopy

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JO - Instrumentation Science and Technology

JF - Instrumentation Science and Technology

IS - 6

ER -

Determination of hydroxypyrene trisulfonate by two wavelength excitation fluorescence using a one microliter capillary

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Keywords

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