TY - JOUR
T1 - Determination of coproporphyrinogen III oxidase activity in bacteria
AU - Mamet, Rivka
AU - Scharf, Ram
AU - Keller, Nathan
AU - Zimmels, Yoram
AU - Schoenfeld, Nili
PY - 1994/3
Y1 - 1994/3
N2 - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.
AB - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.
KW - Coproporphyrinogen III oxidase
KW - E. coli
KW - HPLC
UR - http://www.scopus.com/inward/record.url?scp=0028348848&partnerID=8YFLogxK
U2 - 10.1016/0167-7012(94)90067-1
DO - 10.1016/0167-7012(94)90067-1
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AN - SCOPUS:0028348848
SN - 0167-7012
VL - 19
SP - 173
EP - 178
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 3
ER -
