TY - JOUR
T1 - Determination of absolute values of dipolar cross‐relaxation rates for ligands bound to macromolecules using double‐selective T1
AU - Gaggelli, Elena
AU - Valensin, Gianni
AU - Kushnir, Tamir
AU - Navon, Gil
PY - 1992/6
Y1 - 1992/6
N2 - Measuring double‐selective and single‐selective proton relaxation rates has been shown to provide directly the dipolar cross‐relaxation rate of any pair of protons in a ligand molecule. The same measurements for a ligand exchanging between a free and a macromolecule‐bound state yield the cross‐relaxation rate of the bound ligand provided that the rate of exchange is relatively fast, thus allowing one to obtain dynamic and conformational features of the bound ligand. As an example, rigid binding of sulphisomidine to bovine serum albumin has been demonstrated.
AB - Measuring double‐selective and single‐selective proton relaxation rates has been shown to provide directly the dipolar cross‐relaxation rate of any pair of protons in a ligand molecule. The same measurements for a ligand exchanging between a free and a macromolecule‐bound state yield the cross‐relaxation rate of the bound ligand provided that the rate of exchange is relatively fast, thus allowing one to obtain dynamic and conformational features of the bound ligand. As an example, rigid binding of sulphisomidine to bovine serum albumin has been demonstrated.
KW - Bovine serum albumin
KW - H NMR
KW - Selective relaxation rates
KW - Sulphanilamides
UR - http://www.scopus.com/inward/record.url?scp=84989045682&partnerID=8YFLogxK
U2 - 10.1002/mrc.1260300602
DO - 10.1002/mrc.1260300602
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AN - SCOPUS:84989045682
SN - 0749-1581
VL - 30
SP - 461
EP - 465
JO - Magnetic Resonance in Chemistry
JF - Magnetic Resonance in Chemistry
IS - 6
ER -