TY - JOUR
T1 - Determination of ΔpH in cholinergic synaptic vesicles
T2 - Its effect on storage and release of acetylcholine
AU - Michaelson, Daniel M.
AU - Angel, Itzchak
N1 - Funding Information:
We thank Prof. G. Rudnick, Prof. M. Sokolovsky and Mr. G. Klemperer for their help and valuable discussions; Dr. B. Berger of Hoffman La Roche for the gift of nigericin and Dr. Hytler of Dupont for donating FCCP. This work was supported in part by the Israel Center for Psychobiology and by the Israel Commission for Basic Sciences.
PY - 1980
Y1 - 1980
N2 - The interior of purified cholinergic Torpedo vesicles is acidic, pHin = 5.2 at external pH = 7.4. The internal pH changes linearily as a function of external pH yielding ΔpH = 2.0 and 2.5 at pHout = 6.3 and 9.1 respectively. The proton translocator carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) + the ionophore valinomycin dissipate the proton gradient across the vesicular membrane and concurrently induce acetylcholine release from vesicles suspended in K+ buffer. The effect of FCCP + valinomycin is not sensitive to external pH values between 6.3 and 9.1 and is diminished at lower external pH. The possible role of intravesicular pH and of the proton electrochemical gradient in the storage of acetylcholine within cholinergic vesicles is discussed.
AB - The interior of purified cholinergic Torpedo vesicles is acidic, pHin = 5.2 at external pH = 7.4. The internal pH changes linearily as a function of external pH yielding ΔpH = 2.0 and 2.5 at pHout = 6.3 and 9.1 respectively. The proton translocator carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) + the ionophore valinomycin dissipate the proton gradient across the vesicular membrane and concurrently induce acetylcholine release from vesicles suspended in K+ buffer. The effect of FCCP + valinomycin is not sensitive to external pH values between 6.3 and 9.1 and is diminished at lower external pH. The possible role of intravesicular pH and of the proton electrochemical gradient in the storage of acetylcholine within cholinergic vesicles is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0019308646&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(80)90017-X
DO - 10.1016/0024-3205(80)90017-X
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AN - SCOPUS:0019308646
SN - 0024-3205
VL - 27
SP - 39
EP - 44
JO - Life Sciences
JF - Life Sciences
IS - 1
ER -