@article{b77da9b7edba4fb38df61b914459b297,
title = "Determination of Δψ, ΔpH and the proton electrochemical gradient in isolated cholinergic synaptic vesicles",
abstract = "The electrical potential (Δψ) of intact cholinergic synaptic vesicles was measured in the presence and absence of the proton translocator carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP), and the results were utilized to calculate the vesicular proton chemical gradient (ΔpH) and proton electrochemical potential (ΔμH+). At external pH = 7.4 the vesicles maintain a proton electrochemical gradient of {reversed tilde equals}+20 mV (positive inside) which is composed of Δψ{reversed tilde equals}-80 mV (negative inside) and ΔpH{reversed tilde equals}1.6 (acidic inside). The proton chemical gradient (ΔpH) increases as a function of pHout whereas the vesicular electrical potential (Δψ) is only slightly affected by the external pH. Consequently, ΔμH+ is larger at basic external pH values ({reversed tilde equals}+40 mV at pHout = 9.0) and smaller at acidic external pH values (ΔμH+{reversed tilde equals}0 at (pHout = 5.6). The possible physiological role of the electrochemical potentials in maintaining high concentrations of acetylcholine within the cholinergic synaptic vesicle is discussed.",
author = "Itzchak Angel and Michaelson, {Daniel M.}",
note = "Funding Information: We thank Prof. M. Sokolovsky and Dr. I. Friedberg for their help and valuable discussions and Dr. Hytler of Dupont for donating FCCP. This work was supported in part by a grant from the Muscular Dystrophy Association.",
year = "1981",
month = jul,
day = "27",
doi = "10.1016/0024-3205(81)90335-0",
language = "אנגלית",
volume = "29",
pages = "411--416",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "4",
}